Y0503

Y-27632 dihydrochloride

≥98% (HPLC)

• 동의어(들): Rock inhibitor y-27632, Y-27632 2HCl, (R)-(+)-trans-4-(1-Aminoethyl)-N-(4-Pyridyl)cyclohexanecarboxamide dihydrochloride
• 실험식(Hill 표기법): C₁₄H₂₁N₃O · 2HCl
• CAS Number: 129830-38-2
• Molecular Weight: 320.26
• MDL number: MFCD03490488
• UNSPSC 코드: 12352200
• PubChem Substance ID: 24277699
• NACRES: NA.77

속성

• 생물학적 소스: synthetic (organic)
• Quality Level: 100
• 분석: ≥98% (HPLC)
• 양식: powder
• 광학 활성: [α]/D +3.0 to +5.0°, c = 1.0 in methanol
• 색상: white to beige
• solubility: H₂O: 14 mg/mL
• 저장 온도: 2-8°C
• SMILES string: Cl.Cl.C[C@@H](N)[C@H]1CC[C@@H](CC1)C(=O)Nc2ccncc2
• InChI: 1S/C14H21N3O.2ClH/c1-10(15)11-2-4-12(5-3-11)14(18)17-13-6-8-16-9-7-13;;/h6-12H,2-5,15H2,1H3,(H,16,17,18);2*1H/t10-,11-,12-;;/m1../s1
• InChI key: IDDDVXIUIXWAGJ-LJDSMOQUSA-N
• 유전자 정보: human ... ROCK1(6093) , ROCK2(9475)

설명

일반 설명

Y-27632 dihydrochloride is an effective bronchodilator and improves lung resistance (RL) induced by antigen and acetylcholine.[1] Y-27632 controls neutrophil migration and lung edema and may be a potential agent against acute lung injury.[2]The utilization of Y-27632 dihydrochloride, in conjunction with Accutase or other enzyme solutions, during the passaging of human embryonic stem (hES) cells has been demonstrated to enhance cell survival and significantly improve cloning efficiency[3]

Y-27632, a Rho-kinase inhibitor, counteracts noradrenergic contractions in the penile corpus cavernosum of rabbits and humans. It helps to relax vascular and non-vascular smooth muscle in various preparations and lower blood pressure in hypertensive rats.[4] Additionally, this inhibitor induces reversible changes in cell shape and decreases in actin stress fibers, focal adhesions, and protein phosphotyrosine staining in human trabecular meshwork (TM) cells and Schlemm′s canal cells.[5] Y-27632 inhibits the kinase activity of both ROCK-I and ROCK-II in vitro, and this inhibition is competitively reversed by ATP, indicating that these compounds bind to the catalytic site to inhibit the kinases.[6] Ki = 140 nM for p160ROCK.

애플리케이션

Y-27632 dihydrochloride has been used:

• as a medium supplement in pancreatic ductal adenocarcinoma organoid culture[7]
• in the inhibition of Ras homolog gene family (Rho) kinase in mouse embryonic stem cells[8]
• in the inhibition of Rho-associated protein kinase (ROCK) in human embryonic stem cells[9] and human induced pluripotent stem cells (iPSCs)[10]
• Y-27632 dihydrochloride has been used as a supplement in the E8 medium to promote in vitro differentiation of human pluripotent stem cells[11]

생화학적/생리학적 작용

Y-27632 is a highly potent, cell-permeable, selective ROCK inhibitor.

Y-27632 is highly potent, cell-permeable, selective ROCK (Rho-associated coiled coil forming protein serine/threonine kinase) inhibitor. K_i = 140 nM for p160^ROCK. Y-27632 also inhibits ROCK-II with equal potency. The inhibition is competitive with respect to ATP.

특징 및 장점

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법적 정보

Sold under license from Mitsubishi Pharma Corporation

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Gloves