It is a good antibody for Western Blot for both specimen of human and mouse. But it often shows signals medium intensity, not strong enough.
추천 제품
생물학적 소스
rabbit
결합
unconjugated
항체 형태
IgG fraction of antiserum
항체 생산 유형
primary antibodies
클론
polyclonal
기술
immunocytochemistry: 5 μg/mL using V5 tagged fusion protein in methanol-acetone fixed transiently transfected 293T cells
immunoprecipitation (IP): 5 μg/mL using V5 tagged fusion protein from extracts of transfected cells
western blot: 2.5 μg/mL using V5 tagged fusion protein expressed in whole extracts of transfected cells
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
일반 설명
V5 is an amino coterminal protein consisting of a C-terminus cysteine-rich region that resembles a cysteine-rich metal binding domain. It is encoded by the "P" gene of the paramyxovirus SV5.
The rabbit Anti-V5 synthetic peptide antibody reacts with V5 tagged recombinant fusion proteins expressed in transfected mammalian cells and from in vitro translation.
The rabbit Anti-V5 synthetic peptide antibody reacts with V5 tagged recombinant fusion proteins expressed in transfected mammalian cells and from in vitro translation.
특이성
Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins
애플리케이션
Anti-V5 antibody produced in rabbit has been used in:
- immunocytochemistry
- immunoprecipitation
- western blot
물리적 형태
Solution in 0.01M phosphate buffered saline, pH 7.4 containing 15 mM sodium azide.
저장 및 안정성
Store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
면책조항
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
적합한 제품을 찾을 수 없으신가요?
당사의 제품 선택기 도구.을(를) 시도해 보세요.
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Pyk2 is a Novel Tau Tyrosine Kinase that is Regulated by the Tyrosine Kinase Fyn
Chuanzhou Li and Jurgen G
Journal of Alzheimer's disease reports, 64(1), 205-221 (2018)
Fredrik Holmström et al.
Scientific reports, 6, 24991-24991 (2016-05-05)
The hepatitis C virus nonstructural (NS) 3/4A and NS5A proteins are major targets for the new direct-acting antiviral compounds. Both viral proteins have been suggested as modulators of the response to the host cell. We have shown that NS3/4A- and
Mutations in MAPKBP1 Cause Juvenile or Late-Onset Cilia-Independent Nephronophthisis
Macia MS, et al.
American Journal of Human Genetics, 100(2), 323-333 (2017)
Bente B Johansson et al.
The Journal of biological chemistry, 286(40), 34593-34605 (2011-07-26)
CEL-maturity onset diabetes of the young (MODY), diabetes with pancreatic lipomatosis and exocrine dysfunction, is due to dominant frameshift mutations in the acinar cell carboxyl ester lipase gene (CEL). As Cel knock-out mice do not express the phenotype and the
S M Thomas et al.
Cell, 54(6), 891-902 (1988-09-09)
The "P" gene of the paramyxovirus SV5 encodes two known proteins, P (Mr approximately equal to 44,000) and V (Mr approximately equal to 24,000). The complete nucleotide sequence of the "P" gene has been obtained and is found to contain
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
