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주요 문서

U8501

Sigma-Aldrich

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast

Type X, lyophilized powder, ≥40 units/mg protein

동의어(들):

UTP:α-D-glucose-1-phosphate uridylyltransferase

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25 UNITS
₩286,535
100 UNITS
₩875,235
500 UNITS
₩2,341,707
1000 UNITS
₩3,983,774

₩286,535


구입 가능 여부는 고객센터에 문의하십시오.


크기 선택

보기 변경
25 UNITS
₩286,535
100 UNITS
₩875,235
500 UNITS
₩2,341,707
1000 UNITS
₩3,983,774

About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

₩286,535


구입 가능 여부는 고객센터에 문의하십시오.

생물학적 소스

bakers yeast

Quality Level

유형

Type X

양식

lyophilized powder

특이 활성도

≥40 units/mg protein

구성

Protein, 30-60% modified Warburg-Christian

외래 활성

UDP-glucose dehydrogenase and galactose-1-phosphate uridyltransferase ≤0.1%
inorganic pyrophosphatase ≤0.5%

저장 온도

−20°C

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일반 설명

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) is ubiquitous in plants, yeast, bacteria, and mammals. This enzyme is an octamer of eight identical sub-units.[1]

애플리케이션

Uridine-5′-diphosphoglucose pyrophosphorylase from baker′s yeast has been used:
  • in the synthesis of uridine-5′-diphosphoglucose (UDP-Glc)-13C9[2]
  • to quantify Suc synthase (SUS) enzyme activity in rice[3]
  • to study the role of hexokinase and glycogen synthase controls the flux in frog oocytes[4]

생화학적/생리학적 작용

Uridine-5′-diphosphoglucose pyrophosphorylase (UDP-glc-PPase) participates in catalyzing the synthesis of UDP-glucose. This enzyme requires divalent cations such as Mg2+, Ca2+, Mn2+, Ni2+ for its activity.[1]

단위 정의

One unit will form 1.0 μmole of glucose 1-phosphate from uridine-5′-diphosphoglucose and inorganic pyrophosphate per min at pH 7.6 at 25 °C.

물리적 형태

Lyophilized, sulfate-free powder containing citrate buffer salt

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

Yonglan Yu et al.
PloS one, 5(9), e12593-e12593 (2010-09-11)
The trehalose synthetic pathway is present in bacteria, fungi, plants and invertebrate animals, but is absent in vertebrates. This disaccharide mainly functions as a stress protectant against desiccation, heat, cold and oxidation. Genes involved in trehalose synthesis have been observed
Jesús Rodríguez-Díaz et al.
Journal of biotechnology, 154(4), 212-215 (2011-06-15)
UDP-sugars are widely used as substrates in the synthesis of oligosaccharides catalyzed by glycosyltransferases. In the present work a metabolic engineering strategy aimed to direct the carbon flux towards UDP-glucose and UDP-galactose biosynthesis was successfully applied in Lactobacillus casei. The
Himangi R Jayakar et al.
BMC microbiology, 11, 179-179 (2011-08-09)
A number of studies have revealed that Francisella tularensis (FT) suppresses innate immune responses such as chemokine/cytokine production and neutrophil recruitment in the lungs following pulmonary infection via an unidentified mechanism. The ability of FT to evade early innate immune
D J Sutor et al.
Clinica chimica acta; international journal of clinical chemistry, 86(3), 329-332 (1978-06-15)
The concentration of pyrophosphate in urine has been determined using the enzyme uridine-5'-diphosphoglucose pyrophosphorylase. The technique is relatively quick and easy to perform. Reproducibility of results was good, and results from recovery experiments were excellent. The mean concentration of pyrophosphate
Laura Bonofiglio et al.
Microbial drug resistance (Larchmont, N.Y.), 17(1), 75-81 (2010-12-07)
Prevalence of serotype 6B penicillin (PEN)-nonsusceptible Streptococcus pneumoniae significantly increased from 15.8% (1993-1997) to 67.3% (1998-2002) (p<0.001) in Argentina. Serogroup 6 ranks fourth among different capsular types within invasive isolates from Argentinean patients <6 years of age. To evaluate whether

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