T3573

RANK Ligand/TRANCE human

>90% (SDS-PAGE), recombinant, expressed in NSO cells, lyophilized powder

• 동의어(들): Osteoclast Differentiation Factor (ODF), Osteoprotegerin Ligand (OPGL), Receptor Activator of NF-KB Ligand (RANKL), TNF-related activation-induced cytokines (TRANCE)
• CAS Number: 207621-35-0
• MDL number: MFCD01867142
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in NSO cells
• 분석: >90% (SDS-PAGE)
• 형태: lyophilized powder
• 효능: 1.5-7.5 ng/mL ED₅₀
• 분자량: glycolysylated recombinant product ~35 kDa by SDS-PAGE; calculated mol wt 23 kDa
• 포장: pkg of 10 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 불순물: endotoxin, tested
• UniProt 수납 번호: O14788
• 응용 분야: cell analysis
• 저장 온도: −20°C
• 유전자 정보: human ... TNFSF11(8600)

설명

일반 설명

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) is also known as receptor activator of nuclear factor-κB ligand (RANKL). It is a type II transmembrane signaling receptor. It has a molecular weight of around 35kDa. This protein contains an amino-terminal intracellular tail and a carboxy-terminal extracellular region, that bears a connecting stalk and a receptor-binding domain. Tnfsf11 is located on human chromosome 13q14.11.

애플리케이션

RANK Ligand/TRANCE human can be used in in vitro osteoclastogenic assays for osteoclast differentiation.

RANK Ligand/TRANCE human has been used to stimulate differentiation.

생화학적/생리학적 작용

RANK Ligand (receptor activator of NF-kB ligand (RANKL) also referred to as TNF-related activation induced cytokines (TRANCE) or osteoprotegerin ligand is a protein that belongs to tumor necrosis factor (TNF) family. It stimulates the mature dendritic cells and induces the cytokine production. RANKL also facilitates bone remodelling and possess an angiogenic activity.

RANK Ligand/TRANCE, a member of the TNF superfamily, induces activation of the c-jun N-terminal kinase, enhances T-cell growth and dendritic cell function, induces osteoclastogenesis, and lymph node organogenesis. Mouse and human RANK Ligand share 85% amino acid identity. RANK is the cell surface receptor for RANK Ligand.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in 20 mM MOPS and 500 mM NaCl, pH 6.5, with 50 μg BSA per 1 μg as a carrier protein.

분석 메모

The biological activity is measured by its ability to induce osteoclast differentiation on mouse splenocytes.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)