R0525

RANK Ligand from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 동의어(들): Osteoclast differentiation factor (ODF), Osteoprotegerin ligand (OPGL), Receptor activator of NF-κB Ligand (RANKL), TNF-related activation−induced cytokines (TRANCE)
• MDL number: MFCD01867142
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: ≥98% (SDS-PAGE)
• 양식: lyophilized powder
• 효능: 10.0-25.0 ng/mL ED₅₀
• 포장: pkg of 10 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: <0.1 ng/mg; <1 EU/μg
• 색상: white
• UniProt 수납 번호: O35235
• 저장 온도: −20°C
• 유전자 정보: mouse ... Tnfsf11(21943)

설명

일반 설명

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or receptor activator of nuclear factor-κB ligand (RANKL) is a type II transmembrane signaling receptor. It has a molecular weight of about 35kDa. The protein possesses an amino-terminal intracellular tail and a carboxy-terminal extracellular region, which has a connecting stalk and a receptor-binding domain.[1][2]

애플리케이션

RANK ligand from mouse has been used to visualize differentiated osteoclasts and to stimulate osteoclast differentiation.[3]

The functions of RANK Ligand include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function induction of osteoclastogenesis, and lymph node organogenesis.The cell surface signaling receptor of RANK Ligand is RANK, which undergoes receptor clustering during signal transduction.

생화학적/생리학적 작용

Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or RANKL has an important role in osteoclast function and differentiation. It binds to RANK and osteoprotegrin (OPG).[1][2] Bioactivities of RANKL may be attributed to its binding to these two receptor molecules. Mouse and human RANKL share 85% amino acid sequence identity.

물리적 형태

Lyophilized from a 0.2 μm filtered solution of 5 mM sodium phosphate, pH 7.6, and 75 mM NaCl.

분석 메모

The bioactivity is determined by its ability to induce osteoclast differentiation of RAW 264.7. The expected ED₅₀ for this effect is ≤10 ng/ml.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)