SRP3267

NT-3 human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• 동의어(들): HGNF, NGF-2, Nerve growth factor-2
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 양식: lyophilized
• 효능: 20-50 ng/mL ED₅₀
• 분자량: 27.2 kDa
• 포장: pkg of 10 μg
• 불순물: <0.1 EU/μg endotoxin, tested
• 색상: white to off-white
• UniProt 수납 번호: P20783
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... NTF3(4908)

설명

일반 설명

NTF3 (neurotrophin 3) is a neurotrophic factor belonging to the NGF (nerve growth factor) family.[1] This protein is composed of 257 amino acids, with a prepro region of 138 amino acids and the mature sequence of 119 residues.[2]

생화학적/생리학적 작용

NT3 functions on two structurally unrelated receptor types- the p75 neurotrophin receptor (p75^NTR) and the receptor tyrosine kinase (TRK). It promotes the metastatic invasion of brain by breast cancer cells, by promoting the EMT (epithelial-mesenchymal transition) transition of breast cancer cells and suppressing the immune response in brain.[1] This protein is responsible for the indction and survival of neurite outgrowth from a subset of neural crest (NC) and placode-derived neurons.[3]
Recombinant human NT-3 is a noncovalently linked homodimer, of two 13.6kDa polypeptide monomers (240 total amino acid residues).

물리적 형태

Lyophilized with no additives.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable