SRP3083

IL-1 beta human

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 동의어(들): Catabolin, Endogenous Pyrogen (EP),, Leukocyte Endogenous Mediator (LEM),, Lymphocyte-activating factor (LAF), Mononuclear Cell Factor (MCF)
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 양식: lyophilized
• 효능: ≤0.001 ng/mL ED₅₀
• 분자량: 17.3 kDa
• 포장: pkg of 10 μg
• 기술: cell culture | mammalian: suitable
• 불순물: <0.1 EU/μg endotoxin, tested
• 색상: white to off-white
• UniProt 수납 번호: P01584
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... IL1B(3553)

설명

일반 설명

IL-1β (interleukin 1 β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells.[1][2] It is synthesized as a pro-IL-1β which is changed to active form IL-1β with the help of pro-inflammatory protease caspase-1.[3] IL-1β belongs to the IL-1 gene family.[4] The gene is mapped to human chromosome 2q14.[5] Recombinant human IL-1β is a 17.3kDa protein containing 153 amino acid residues.

애플리케이션

IL-1β (interleukin 1 β) human has been used to study the effect of pro- and anti-inflammatory mediators on leukotriene B4 and C4 synthesis as well as secretion in an inflamed porcine endometrium.[6] It has been used to study the effect of cytokines on synthesis and secretion of leukotrienes from endometrial epithelial cells of pigs.[7]

생화학적/생리학적 작용

Both IL-1α (interleukin 1 α) and IL-1β binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF (fibroblast growth factor)-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells.[8][9][2][4] However, whereas IL-1β is a secreted cytokine, IL-1α is predominantly a cell-associated cytokine.[10] IL-1β is involved in proinflammatory and immunological responses. Some of them include enhancing IL-2 generation, and increasing circulating NO (nitric oxide) and bringing neutrophils. It also regulates gene expression of inflammatory cytokines, mesenchymal growth factors and colony stimulating factors.[1] IL-1β is present in chronic inflamed tissues and also in tissues going for fibrogenesis.[4] It brings neutrophil and macrophage to the site of infection.[11] Mutations in IL-1β gene are linked with renal manifestations and renal sequelae in Henoch-Schönlein purpura.[12] It is also associated with destruction of β-cell in type 1 diabetes.[13]

서열

APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS

물리적 형태

Lyophilized from 10 mM Sodium Phosphate, pH 7.4 + 50mM NaCl.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable