S9396

Sphingomyelinase from Bacillus cereus

buffered aqueous glycerol solution, ≥100 units/mg protein (Lowry)

• 동의어(들): Sphingomyelin choline phosphohydrolase, Sphingomyelin phosphodiesterase
• CAS Number: 9031-54-3
• 효소 위원회 번호: 3.1.4.12 (BRENDA, IUBMB)
• MDL number: MFCD00132362
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: buffered aqueous glycerol solution
• Quality Level: 200
• 특이 활성도: ≥100 units/mg protein (Lowry)
• 분자량: 34.2 kDa
• 외래 활성: Phospholipase C ≤1%
• 저장 온도: 2-8°C
• 유전자 정보: Bacillus cereus ATCC 10987 ... spH(2751530)

설명

일반 설명

Sphingomyelinase (SMase) is a hydrolase,[1] of 34.2 kDa and its active site contain as a minimum of two magnesium atoms.[2]

애플리케이션

Sphingomyelinase from Bacillus cereus has been used:

• in the study to analyse the effect of sphingomyelinase on Rubella virus infectivity in cell lines[3]
• in lipoprotein modification for the generation of cholesterol crystals in vitro[4]
• to aggregate LDL (low-density lipoprotein)[5]

Sphingomyelinase has been used in a study to assess the interaction of actin with the HIV-1 accessory protein Nef. [6] Sphingomyelinase has also been used in a study to investigate X-ray scattering as a quality-control tool for liposomal drug-delivery systems. [7]

생화학적/생리학적 작용

Initiates the formation of sphingomyelin-based second messengers. Activates MAPK (mitogen-activated protein kinase) and SAPKs (stress-activated protein kinases); generates ceramide from sphingomyelin.

Sphingomyelin phosphodiesterase (SMPD) participates in the metabolic reactions comprising sphingomyelin.[1] SMPD has the ability to prevent colorectal carcinoma and colitis.[1]

단위 정의

One unit will hydrolyze 1.0 μmole of TNPAL-sphingomyelin per min at pH 7.4 at 37 °C.

물리적 형태

Solution in 50% glycerol containing 50 mM Tris-HCl, pH 7.5.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable