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Merck
모든 사진(1)

주요 문서

P4912

Sigma-Aldrich

Phospholipase D from Streptomyces sp.

Type VII, lyophilized powder, ≥150 units/mg solid

동의어(들):

Lecithinase D, Phosphatidylcholine phosphatidohydrolase

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크기 선택

250 UNITS
₩512,677
500 UNITS
₩839,811
1000 UNITS
₩1,545,947

₩512,677


구입 가능 여부는 고객센터에 문의하십시오.


크기 선택

보기 변경
250 UNITS
₩512,677
500 UNITS
₩839,811
1000 UNITS
₩1,545,947

About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

₩512,677


구입 가능 여부는 고객센터에 문의하십시오.

생물학적 소스

Streptomyces sp.

Quality Level

유형

Type VII

양식

lyophilized powder

특이 활성도

≥150 units/mg solid

저장 온도

−20°C

애플리케이션

Phospholipase D (PLD) has been used to hydrolyze the phosphate bonds of phospholipids and sphingomyelin to yield the corresponding phosphatidic acid. It has also been used to study metabolic labeling and direct imaging of choline phospholipids in vivo by measuring propargyl-Cho incorporation. Furthermore, PLD has been used in purification and kinetic studies.
The enzyme from Sigma has been used in the translocation of sphingosine kinase 1 (SK1) to membrane fractions under in vitro conditions.[1] It has also been used to produce phosphatidic acid (PA) from phosphatidylcholine (PC) in HL60 permeabilized cells.[2]

생화학적/생리학적 작용

Hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.
Phospholipase D is glycerophospholipid-specific. It is markedly less active on sphingomyelins and lysophospholipids. Phospholipase D hydrolyzes the phosphate bonds of phospholipids and sphingomyelin to give the corresponding phosphatidic acid.

포장

Bottomless glass bottle. Contents are inside inserted fused cone.

단위 정의

One unit will liberate 1.0 μmole of choline from 1,2-Dioleoyl-sn-glycero-3-phosphocholine per hour at pH 8.0 at 37 °C.

분석 메모

Protein by biuret

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

예방조치 성명서

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

Fava beans can substitute soybean meal and rapeseed meal as protein source in diets for lactating dairy cows.
Hansen, et al.
Journal of Dairy Science, 104, 5508-5521 (2021)
Mohamed H Yaghmour et al.
Journal of lipid research, 62, 100022-100022 (2021-01-17)
Phospholipids with a choline head group are an abundant component of cellular membranes and are involved in many important biological functions. For studies on the cell biology and metabolism of these lipids, traceable analogues where propargylcholine replaces the choline head
Christine Delon et al.
The Journal of biological chemistry, 279(43), 44763-44774 (2004-08-18)
Sphingosine kinase 1 (SK1) phosphorylates sphingosine to generate sphingosine 1-phosphate (S1P). Because both substrate and product of the enzyme are potentially important signaling molecules, the regulation of SK1 is of considerable interest. We report that SK1, which is ordinarily a
Kathryn Garner et al.
The Journal of biological chemistry, 287(38), 32263-32276 (2012-07-24)
Phosphatidylinositol transfer proteins (PITPs) are versatile proteins required for signal transduction and membrane traffic. The best characterized mammalian PITPs are the Class I PITPs, PITPα (PITPNA) and PITPβ (PITPNB), which are single domain proteins with a hydrophobic cavity that binds
Milya Davlieva et al.
Antimicrobial agents and chemotherapy, 57(1), 289-296 (2012-11-02)
Daptomycin (DAP) resistance in enterococci has been linked to mutations in genes that alter the cell envelope stress response (CESR) (liaFSR) and changes in enzymes that directly affect phospholipid homeostasis, and these changes may alter membrane composition, such as that

질문

  1. 本製品はカルタヘナ法に該当する試薬でしょうか。また菌体除去のためのフィルターろ過はされているのでしょうか。

    1 답변
    1. The Cartagena Protocol is not applicable to this product. While the manufacuturing process is proprietary, current methods of extraction and purification would eliminate the presence of any whole, vialble organisms. This is not a sterile product. Prepared solutions may be filter-sterilized prior to use.

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