S8160

Superoxide Dismutase from bovine liver

lyophilized powder, ≥1500 units/mg protein

• 동의어(들): SOD, Superoxide: superoxide oxidoreductase
• CAS Number: 9054-89-1
• 효소 위원회 번호: 1.15.1.1 (BRENDA, IUBMB)
• EC Number: 232-943-0
• MDL number: MFCD00132404
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: lyophilized powder
• 특이 활성도: ≥1500 units/mg protein
• 분자량: 32.5 kDa
• 구성: Protein, ≥70% biuret
• UniProt 수납 번호: P00442
• 저장 온도: −20°C
• 유전자 정보: cow ... SOD1(281495)

설명

일반 설명

Research area: Cell signalingSuperoxide Dismutase (SOD), a low molecular weight protein that is found in all aerobic cells of microorganisms, plants, and animals.[1] It exists as three distinct families such as manganese SOD, copper–zinc SOD, and extracellular SOD.[1][2]

애플리케이션

Superoxide dismutase from bovine liver has been used in a study to determine that hypercholesterolemia increases endothelial superoxide anion production. [3] Superoxide dismutase from bovine liver has also been used in a study to investigate diazo coupling, subunit interactions, and electrophoretic variants of bovine erythrocyte superoxide dismutase. [4]It has also been used as a component of the assay buffer in thequantification of reactive oxygen species (ROS) by O2k fluorometry.[5]

생화학적/생리학적 작용

Superoxide Dismutase catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It plays a critical role in the defense of cells against the toxic effects of oxygen radicals. It competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

단위 정의

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀ of 0.025 ± 0.005 per min.

물리적 형태

Lyophilized powder containing potassium phosphate buffer salts

분석 메모

For assay method, see McCord, J.M. and Fridovich, I., J. Biol. Chem., 244, 6049 (1969).

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)