S5395

Superoxide Dismutase from bovine erythrocytes

BioReagent, ≥3,000 units/mg protein, lyophilized powder, suitable for cell culture

• 동의어(들): SOD, Superoxide: superoxide oxidoreductase
• CAS Number: 9054-89-1
• 효소 위원회 번호: 1.15.1.1 (BRENDA, IUBMB)
• EC Number: 232-943-0
• MDL number: MFCD00132404
• UNSPSC 코드: 12352204
• NACRES: NA.75

속성

• 생물학적 소스: bovine erythrocytes
• 제품 라인: BioReagent
• 형태: lyophilized powder
• 특이 활성도: ≥3,000 units/mg protein
• 분자량: 32.5 kDa
• 포장: pkg of 15000 units
• 기술: cell culture | mammalian: suitable
• pH: 7.6-10.5
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Superoxide Dismutase from bovine erythrocytes is a metalloprotein which disproportionates superoxide anion radicals. It is a 31.5 kDa copper binding protein and displays a conserved domain and fold. It is a homodimer with one copper and zinc ion per subunit and has antiparallel “greek-key” β barrel fold.

애플리케이션

Superoxide Dismutase (SOD) from bovine erythrocytes has been used:

• for measuring the superoxide radical using the electron paramagnetic resonance spin in human brain microvascular endothelial cells
• for measuring superoxide production in cytochrome C assay in peripheral blood mononuclear cells
• as a standard in characterization of hen egg SOD using Fourier-transform infrared spectroscopy (FTIR) and matrix-assisted laser desorption/ionization (MALDI) analysis

생화학적/생리학적 작용

Superoxide Dismutase from bovine erythrocytes catalyzes the dismutation of superoxide radicals to hydrogen peroxide and molecular oxygen. It serves as an antioxidant and plays a critical role in the defense of cells against the toxic effects of oxygen radicals. Competes with nitric oxide (NO) for superoxide anion (which reacts with NO to form peroxynitrite), thereby SOD promotes the activity of NO. SOD has also been shown to suppress apoptosis in cultured rat ovarian follicles, neural cell lines, and transgenic mice.

단위 정의

One unit will inhibit reduction of cytochrome c by 50% in a coupled system with xanthine oxidase at pH 7.8 at 25 °C in a 3.0 mL reaction volume. Xanthine oxidase concentration should produce an initial ΔA₅₅₀ of 0.025 ± 0.005 per min.

분석 메모

For assay method, see McCord, J.M. and Fridovich,I., J. Biol. Chem., 244, 6049 (1969).

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)