P8804

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

buffered aqueous glycerol solution, ≥1,000 units/mg protein (Lowry)

• 동의어(들): 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
• CAS Number: 37288-19-0
• 효소 위원회 번호: 4.6.1.13 (BRENDA, IUBMB)
• EC Number: 232-638-2
• MDL number: MFCD00131896
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 생물학적 소스: Bacillus sp. (Bacillus cereus)
• Quality Level: 200
• 양식: buffered aqueous glycerol solution
• 특이 활성도: ≥1,000 units/mg protein (Lowry)
• 분자량: 28 kDa
• 외래 활성: Phopholipase C (lecithinase) ≤1 units/mg protein; Sphingomyelinase ≤40 units/mg protein
• 저장 온도: 2-8°C
• 유전자 정보: Bacillus cereus E33L ... BCZK3513(3026815)

설명

일반 설명

Phospholipase C (PI-PLC) from Bacillus cereus has irregular triosephosphate isomerase (TIM)-barrel structure with eight-standard parallel β barrel.[1] It is a 28 kDa protein.[2]

애플리케이션

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used:

• in the hydrolysis of substrates p-nitrophenylphosphorylcholine (p-NPPC) and p-nitrophenylphosphorylphosphate (p-NPP)[3]
• to cleave glycosylphosphatidylinositol (GPI) anchor of lynx1 protein and its detachment from plasma membrane[4]
• to cleave immunolabeled HeLa cells[5]

생화학적/생리학적 작용

Phospholipase C (PI-PLC) releases diacylglycerol by cleaving the glycosylphosphatidylinositol.[1] It has broad substrate specificity and its activity is influenced by metal ions and surfactants.[2]

Used for the release of GPI anchored proteins from the membrane.

단위 정의

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

물리적 형태

Solution in 60% (v/v) glycerol containing 10 mM Tris-HCl, pH 8.0 and 10 mM EDTA

분석 메모

Acetylcholinesterase is measured according to Ellman, et al.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)