P5542

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus

lyophilized powder, ≥1,000 units/mg protein (Lowry)

• 동의어(들): 1-Phosphatidyl-D-myo-inositol inositol phosphohydrolase, cyclic-phosphate forming, PI-PLC
• CAS Number: 37288-19-0
• 효소 위원회 번호: 4.6.1.13 (BRENDA, IUBMB)
• EC Number: 232-638-2
• MDL number: MFCD00131896
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 형태: lyophilized powder
• Quality Level: 300
• 특이 활성도: ≥1,000 units/mg protein (Lowry)
• 외래 활성: Phopholipase C (lecithinase) ≤1 units/mg protein; Sphingomyelinase ≤40 units/mg protein
• 저장 온도: −20°C
• 유전자 정보: Bacillus cereus E33L ... BCZK3513(3026815)

설명

일반 설명

Phosphatidylinositol-specific phospholipase C (PI-PLC) from Bacillus cereus is a 298 residual protein. It belongs to the class of ubiquitous enzymes. PI-PLC is characterized with a single globular domain and it folds into eight (βα) barrel sheets, out of which only six βα units are found in barrel, lacking helices between strands IV and V and V and VI.

애플리케이션

Phospholipase C, Phosphatidylinositol-specific from Bacillus cereus has been used to cleave GPI-anchored protein from green fluorescent protein- glycosylphosphatidylinositol (GFP-GPI) from the cell membrane. It has also been used in invasion assay to test the involvement of PLAP in T. cruzi invasion to human epithelial type 2 (HEp-2) cells.

생화학적/생리학적 작용

Used for the release of GPI anchored proteins from the membrane.

단위 정의

One unit will liberate one unit of acetylcholinesterase per minute from a membrane-bound crude preparation at pH 7.4 at 30 °C (10 minute incubation).

물리적 형태

Contains phosphate buffer salts, EDTA and stabilizer

분석 메모

Acetylcholinesterase is measured according to Ellman, et al.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)