추천 제품
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
MC5, monoclonal
분자량
antigen 80 kDa
포함
15 mM sodium azide
종 반응성
rat, bovine, mouse, human
기술
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1:500 using rat brain cytosol preparation
동형
IgG2a
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... PRKCB(5579) , PRKCZ(5590)
mouse ... Prkcb(18751) , Prkcc(18752)
rat ... Prkcb(25023) , Prkcg(24681)
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일반 설명
Protein Kinase C (PKC) is a phospholipid and calcium dependent enzyme that regulates intracellular signal transduction. Neuronal PKC modulates several functions such as nerve cell excitability, neuronal plasticity, and release of neurotransmitters. PKC inhibitors have been implicated in diabetic retinopathy . Monoclonal Anti-Protein Kinase C (clone MC5) recognizes an epitope located within the amino acid sequence 296-317 at the hinge region (close to or at the trypsin cleavage site) of PKC . The antibody is specific for PKC in humans, bovines, rats and mice. Binding of the antibody to in vitro purified PKC blocks trypsin-mediated partial proteolysis. Phorbol-ester induced down-regulation of the kinase is inhibited by the introduction of Fab fragment of the antibody into a rodent glioma cell line .
Protein kinase C (PKC) is a 77-90kD protein that belongs to serine/threonine protein kinases family. It has a crucial role in cellular regulation, signal transduction and tumor promotion and oncogenesis. Monoclonal Anti-Protein Kinase C (PKC) antibody can be used for identification and localization of PKC in normal and malignant tissues. It can also be used in immunoblotting (diluted 1:500) using rat brain extracts. Mouse Anti-Protein Kinase C (PKC) antibody reacts specifically with PKC polypeptide (80kD). The product has also shown cross reactivity for rat, bovine, mouse and human.
특이성
Reacts with the 80 kDa polypeptide of PKC. Binding of the antibody to purified kinase in vitro, blocks partial proteolysis by trypsin. Introduction of Fab fragments of the antibody into a rodent glioma cell line inhibits phorbol-ester-induced down-regulation of the kinase.
면역원
purified bovine brain protein kinase C
애플리케이션
Monoclonal Anti-Protein Kinase C (PKC) antibody can be used for the localization of protein kinase C by using different immunochemical assays like- dot blot, immunoprecipitation and ELISA. It can also be used as a primary antibody in immunohistochemistry[1] (diluted 1:200) .
Monoclonal Anti-Protein Kinase C (PKC) antibody is suitable for use in immunohistochemistry (frozen sections), immunoblot (80 kD, using bovine brain PKC, extracts of rat glioma and murine NIH 3T3 cell lines), dot blot, ELISA, microarray and immunoprecipitation (rat glioma and human cell extracts/lysates).
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Zi-Li Yu et al.
International journal of molecular medicine, 37(2), 468-474 (2016-01-09)
Lysophosphatidic acid (LPA) is an efficient, bioactive phospholipid involved in various biological processes. In this study, LPA-induced connective tissue growth factor (CTGF/CCN2) expression and the underlying mechanisms were investigated using the MC3T3-E1 cell line. The MC3T3-E1 cells were stimulated with
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BioMed research international, 2018, 2963232-2963232 (2018-06-02)
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M I López Gálvez
Current pharmaceutical biotechnology, 12(3), 386-391 (2010-10-14)
To review current data regarding protein Kinase C beta (PKCß) inhibitors and its efficacy in reducing the burden of diabetic retinopathy (DR) and diabetic macular edema (DME). MEDLINE search (1980-2009) and presentations to major meetings. DR and DME have emerged
Lorenzo Cangiano et al.
PloS one, 2(12), e1327-e1327 (2007-12-20)
Hyperpolarization-activated cyclic nucleotide-sensitive (HCN) channels mediate the I(f) current in heart and I(h) throughout the nervous system. In spiking neurons I(h) participates primarily in different forms of rhythmic activity. Little is known, however, about its role in neurons operating with
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