A3263

Alcohol Dehydrogenase from Saccharomyces cerevisiae

powder, ≥300 units/mg protein, mol wt ~141,000 (four subunits)

• 동의어(들): ADH1, ADH, Alcohol Dehydrogenase from yeast, Alcohol:NAD⁺ oxidoreductase
• CAS Number: 9031-72-5
• 효소 위원회 번호: 1.1.1.1 (BRENDA, IUBMB)
• EC Number: 232-870-4
• MDL number: MFCD00081305
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bakers yeast
• 양식: powder
• 특이 활성도: ≥300 units/mg protein
• 분자량: ~141,000 (four subunits)
• 정제법: crystallization
• 저장 조건: (Tightly closed. Dry)
• 환경친화적 대안 제품 특성: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 색상: beige
• 최적 pH: 8.6-9.0
• 적합성: suitable for recycling micro-assay of β-NAD and β-NADH
• UniProt 수납 번호: A0A3G3NDH9; S5RCH3; S5RK20; S5S176
• 환경친화적 대안 카테고리: Enabling,
• 저장 온도: −20°C

설명

일반 설명

Research area: Neuroscience

Yeast alcohol dehydrogenase 1 (ADH1) belongs to the family of zinc-containing alcohol dehydrogenases. It is a homotetramer with each subunit containing one catalytic domain and coenzyme-binding domain.[1]

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in fuel cell research. For more information see the article in biofiles.

애플리케이션

Alcohol Dehydrogenase from Saccharomyces cerevisiae has been used for the determination of NAD⁺ and NADH concentrations.[2][3][4][5]

생화학적/생리학적 작용

ADH (alcohol dehydrogenase) is one of the first enzymes to be isolated and purified. NAD⁺ is its coenzyme. Three isozymes of yeast ADH, that is, yeast alcohol dehydrogenase-1, 2 and 3 (YADH-1, -2, -3) have been identified. YADH-1 is expressed during anaerobic fermentation, YADH-2 is expressed in the cytoplasm and YADH-3 is localized to the mitochondria. A 141kDa tetramer containing 4 equal subunits. The active site of each subunit contains a zinc atom. Each active site also contains 2 reactive sulfhydryl groups and a histidine residue.

Isoelectric point: 5.4-5.8

Optimal pH: 8.6-9.0

Substrates: Yeast ADH is most active with ethanol and its activity decreases as the size of the alcohol increases or decreases. Branched chain alcohols and secondary alcohols also have very low activity.

K_M (ethanol) = 2.1 × 10^-2 M
K_M (methanol = 1.3 × 10^-1 M
K_M (isopropanol) = 1.4 × 10^-1 M

Inhibitors: Compounds that react with free sulfhydryls, including N-alkylmaleimides and iodoacetamide.
Zinc chelator inhibitors, including 1,10-phenanthroline,
8-hydroxyquinoline, 2,2′-dipyridyl, and thiourea.
Substrate analogue inhibitors, including β-NAD analogs, purine and pyrimidine derivatives, chloroethanol, and fluoroethanol.

Extinction Coefficient: E^1% = 14.6 (water, 280 nm)

Yeast alcohol dehydrogenase 1 (YADH1) catalyzes the conversion of acetaldehyde to ethanol during glucose fermentation pathway.[6] It is also implicated in the production of alcohol from amino acid breakdown via the Ehrlich pathway.[7]

규격

The dried enzyme has been stored for several weeks in a vacuum desiccator with little loss in activity. According to experiments described by A. Kornberg,3 the enzyme can be stored in the frozen state and can be thawed repeatedly without marked loss of activity.

단위 정의

One unit will convert 1.0 μmole of ethanol to acetaldehyde per min at pH 8.8 at 25 °C.

물리적 형태

Solids containing <2% citrate buffer salts

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)