P3818

Protein Disulfide Isomerase from bovine liver

≥100 units/mg protein, lyophilized powder

• 동의어(들): PDI
• CAS Number: 37318-49-3
• 효소 위원회 번호: 5.3.4.1 (BRENDA, IUBMB)
• MDL number: MFCD00163364
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: lyophilized powder
• Quality Level: 200
• 특이 활성도: ≥100 units/mg protein
• 분자량: 107 kDa
• 구성: Protein, ~10% Lowry
• 저장 온도: −20°C
• SMILES string: [S](=O)(=O)(ON)c1c(cc(c(c1)C(=O)O)Cl)Cl
• InChI: 1S/C7H5Cl2NO5S/c8-4-2-5(9)6(16(13,14)15-10)1-3(4)7(11)12/h1-2H,10H2,(H,11,12)
• InChI key: DHUYKLYJBKXDBM-UHFFFAOYSA-N

설명

일반 설명

Protein disulfide isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa with the monomer corresponding to 57 kDa. The isoelectric point (pI) is approximately 4.2.[1] The enzyme is a glycoprotein with 12% total carbohydrate content comprising of mannose, galactose, N-acetyl neuraminic acid (NANA) and 2-acetamido-2-deoxyglucose.[2] PDI is an ubiquitous redox chaperone enzyme. It belongs to the, thioredoxin superfamily and is high conserved.[3]

애플리케이션

Protein Disulfide Isomerase from bovine liver has been used:

• to study the functional role of PDI in parasite infection and the interaction between macrophage PDI and L. chagasi[3]
• in the in vitro translation reaction for the generation of disulfide bonds[4]
• in insulin-disulfide reduction assay and peptide binding assay[5]
• as a positive control in thiol-disulfide oxidoreductase activity assay[6]

생화학적/생리학적 작용

Protein Disulfide Isomerase (PDI) is mainly located in the endoplasmic reticulum (ER), where it assists in protein-folding and thiol-disulfide exchanges.[4] It aids protein refolding in vitro allowing recombinant proteins to achieve their native state.[7]

Protein Disulfide Isomerase(PDI) has the C-terminal ER retention sequence Lys-Asp-Glu-Leu. It has active, intracellular traffic to different cell compartments. PDI supports internalization of Chlamydia, cholera and diphtheria toxins in some hosts. PDI is required for Sindbis virus infection and aids in reducing HIV gp120 protein thiols. PDI facilitates formation of the correct disulfide bonds by promoting rapid reshuffling of disulfide pairings.[8]

포장

Package size based on protein content.

물리적 특성

Protein Disulfide Isomerase (PDI) from bovine liver is a homodimer with a molecular weight of 107 kDa (gel filtration) and the molecular weight of the monomer has been reported at 57 kDA (SDS-PAGE). The enzyme is a glycoprotein with 12% total carbohydrate content, composed of 4.6% mannose, 2.5% galactose, 1.4% NANA, and 3.5% 2-acetamido-2-deoxyglucose.

단위 정의

One unit cause a change in A₆₅₀ of 0.01 per min of a 1.0 mg/mL solution of insulin in the presence of dithiothreitol at pH 7.5 at 25 °C.

물리적 형태

Lyophilized powder containing potassium phosphate buffer salts and stabilizer.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)