N5037

Anti-Neurabin II (C-terminal) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-Neural-tissue Specific F-actin Binding Protein II, Anti-PP1bp134, Anti-Protein Phosphatase 1 Regulatory Subunit 9B, Anti-Spinophilin
• MDL number: MFCD02686629
• UNSPSC 코드: 12352203
• NACRES: NA.43

속성

• 생물학적 소스: rabbit
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen 140 kDa
• 종 반응성: rat, mouse, canine
• 기술: immunoprecipitation (IP): 10-20 μg using rat brain extract (S1 fraction); indirect immunofluorescence: 10-20 using MDCK cells.; microarray: suitable; western blot: 1-2 μg/mL using mouse and rat brain extracts (S1 fraction)
• UniProt 수납 번호: O35274
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PPP1R9B(84687) ; mouse ... Ppp1r9b(217124) ; rat ... Ppp1r9b(84686)

설명

일반 설명

Neurabin II belongs to a family of F-actin-binding proteins, highly enriched in dendritic spines and involved in neurite formation. It consists of an F-actin binding domain at the N-terminus, a single PDZ-interacting-domain and a C-terminal coiled-coil domain.

면역원

synthetic peptide corresponding to amino acids 800-817 located at the C-terminal region of rat neurabin II. The sequence is identical in human and mouse neurabin II and not found in neurabin I.

애플리케이션

Anti-Neurabin II (C-terminal) antibody has been used in

• immunoblotting
• immunoprecipitation
• immunocytochemistry

생화학적/생리학적 작용

Neurabins appear to function as bridging proteins by targeting other proteins to the synapse or by linking plasma membrane proteins to the actin cytoskeleton. Neurabin II binds to several proteins including F-actin, protein phosphatase 1 (PP1), at least two subfamilies of G-protein coupled receptors (GPCRs), the α2 adrenergic receptor (α2AR) subtypes and the D2 dopamine receptor. Neurabin II blocks arrestin function in vitro and in vivo at GPCRs and has been also shown to modulate, in vitro and in vivo, both glutaminergic synaptic transmission and the formation and function of dendritic spines. Neurabin II and neurabin I target PP1 subunits that are highly concentrated in dendritic spines and post-synaptic densities. Phosphorylation of neurabin II modulates its interaction with actin filaments, via the anchoring of the neurabin II-PP1 complex within dendritic spines, thereby contributing to the efficacy and plasticity of synaptic transmission.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable