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Two leaders in genome editing, Sigma-Aldrich and the Wellcome Trust Sanger Institute, have joined forces to make the first ever arrayed lentiviral CRISPR knockout libraries. Based upon validated techniques published in the literature, the Sanger CRISPR libraries will put your lab at the forefront of the race to make the next big discovery.
애플리케이션
Functional Genomics/Screening /Target Validation
Sanger Arrayed Whole Genome Lentiviral CRISPR Library has been used for Functional Genomics/Screening /Target Validation.[1]
특징 및 장점
- Vector: U6-gRNA/PGK-Puro-2A-BFP (gRNA only)
- Simplify the workflow with puromycin selection
- Illuminate CRISPR-expressing cells with BFP
- Flip the expression components into and out of the genome using transposase
Additional Features
- Better, not bigger: Two optimized clones per human gene reduces the time, cost, and scale of screening experiments
- Ready-to-screen: Clones are arrayed in a robotics-friendly 96-well format for high throughput screening; requires plasmid preparation to transfect cells or lentivirus preparation to transduce cells
- Collaborative: Real-time, library validation continues
For detailed information on the Sanger library, click here
포장
96-well plates
성분
Bacteria glycerol stock of gRNA-only 3rd generation lenti-based plasmid vector. 2 knockout clones for every human protein-coding gene. Nearly 40,000 sequence confirmed clones
Request a Quote or More Information
Request a Quote or More Information
물리적 형태
50 μl of bacterial glycerol stocks, 450×96-well plates
기타 정보
This product is for R&D use only, not for drug, household, or other uses. Though the lentiviral transduction particles produced are replication incompetent, it is recommended that they be treated as Risk Group Level 2 (RGL-2) organisms in laboratory handling. Follow all published RGL-2 guidelines for laboratory handling and waste decontamination.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
가장 최신 버전 중 하나를 선택하세요:
Enhancing the genome editing toolbox: genome wide CRISPR arrayed libraries
Emmanouil Metzakopian
Scientific Reports (2017)
문서
Genome-wide screening with optimized gRNAs per gene ensures specific and efficient knockout, controlling time and cost.
프로토콜
Sanger 염기서열분석의 단계 또는 사슬종료법에 대해 알아보고 DNA 염기서열분석이 작동하는 방법, 연구 시 Sanger 염기서열분석 결과를 정확하게 판독하는 방법에 대해서 자세히 알아보십시오.
Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.
활성 필터
자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..
고객지원팀으로 연락바랍니다.
