MITOISO2

Mitochondria Isolation Kit

sufficient for 50 applications (2-5 x 10⁷ cells), isolation of enriched mitochondrial fraction from cells

• 동의어(들): Mitochondrial Extraction Kit, Mitochondrial Purification Kit
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• Quality Level: 200
• 사용: sufficient for 50 applications (2-5 x 10⁷ cells)
• 기술: ELISA: suitable; western blot: suitable
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells.[1] The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.

애플리케이션

Mitochondria Isolation Kit has been used in the isolation of mitochondria from:

• Ewing′s Sarcoma Family of Tumor (ESFT) cells[2]
• blood samples of tuberculosis patients[3]
• human neuroblastoma cells and glioma cells[4]
• myotubes[5]
• HepG2 cells[6]

특징 및 장점

• The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
• The isolated mitochondria will mostly have intact inner and outer membranes.
• Can be used to extract mitochondrial proteins for proteome analysis

기타 정보

• This product is for R&D use only, not for drug, household, or other uses.
• Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
• Use ultrapure water for the preparation of reagents.
• Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.

키트 구성품 역시 별도로 이용 가능함

• C2978 CelLytic^™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mL
• P8340 Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mL
• C0356 Protein Extraction Reagent Type 4 1 bottle

안전성 정보

• 픽토그램: GHS08,GHS07,GHS09
• 신호어: Warning
• 유해 및 위험 성명서: H302,H315,H319,H351,H361fd,H411
• 예방조치 성명서: P202 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): 188.6 °F - closed cup
• Flash Point (°C): 87 °C - closed cup