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Merck
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주요 문서

CPISO

Sigma-Aldrich

Chloroplast Isolation Kit

isolation of intact chloroplasts from leaves

동의어(들):

Chloroplast Purification Kit, Isolation Kit for Chloroplast, Kit for Chloroplast Isolation

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크기 선택

1 KIT
₩1,422,421

₩1,422,421


예상 입고일2025년 4월 07일세부사항



크기 선택

보기 변경
1 KIT
₩1,422,421

About This Item

UNSPSC 코드:
12352200
NACRES:
NA.32

₩1,422,421


예상 입고일2025년 4월 07일세부사항


Quality Level

기술

centrifugation: suitable

배송 상태

wet ice

저장 온도

2-8°C

일반 설명

The Chloroplast Isolation Kit provides an efficient procedure for isolating intact chloroplasts from plant leaves. The procedure involves mechanical cell wall and cell membrane breakage, removal of cell debris and leaf tissue by filtration, collection of total cell chloroplast fraction by centrifugation, and separation of intact chloroplasts from broken chloroplasts using a Percoll® layer or gradient.

애플리케이션

Chloroplast Isolation Kit may be used in chloroplast organelle research to isolate intact chloroplasts from plant leaves for structural and functional studies.

특징 및 장점

  • Specifically formulated buffer - Saves time using easy to follow simple and proven protocols
  • Isolates intact chloroplasts - Useful for studies of such processes as carbon assimilation, electron flow and phosphorylation, metabolic transport or protein targeting
  • Total organelle protein complement - Useful for functional studies, metabolic transport, proteomic profiling, and genomic applications
  • Convenient format - Saves time and reduces waste

적합성

The Chloroplast Isolation Kit has been tested for use with spinach, pea, lettuce, cabbage, mangold, and tobacco.

키트 구성품 역시 별도로 이용 가능함

제품 번호
설명
SDS

  • Chloroplast Isolation Buffer 5x (CIB) 500 mL

  • A8022Bovine Serum Albumin, heat shock fraction, pH 5.2, ≥96% 3 gSDS

  • Chloroplast Isolation Buffer 5x (CIB) 4 ea

  • P4937Percoll®, pH 8.5-9.5 (25 °C), suitable for cell culture 100 mLSDS

Storage Class Code

10 - Combustible liquids


가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number

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이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Yayi Tu et al.
PloS one, 10(8), e0136210-e0136210 (2015-08-27)
Potato virus Y (PVY) is an important plant virus and causes great losses every year. Viral infection often leads to abnormal chloroplasts. The first step of chloroplast division is the formation of FtsZ ring (Z-ring), and the placement of Z-ring
Juan Manuel Pérez-Ruiz et al.
The Plant cell, 18(9), 2356-2368 (2006-08-08)
One of the mechanisms plants have developed for chloroplast protection against oxidative damage involves a 2-Cys peroxiredoxin, which has been proposed to be reduced by ferredoxin and plastid thioredoxins, Trx x and CDSP32, the FTR/Trx pathway. We show that rice
Yuexia Wang et al.
BMC plant biology, 20(1), 96-96 (2020-03-07)
The perturbance of chloroplast proteins is a major cause of photosynthesis inhibition under drought stress. The exogenous application of 5-aminolevulinic acid (ALA) mitigates the damage caused by drought stress, protecting plant growth and development, but the regulatory mechanism behind this
Bruna Marques Dos Santos et al.
Journal of proteomics, 150, 252-257 (2016-10-25)
Photosynthetic organisms may be drastically affected by the future climate projections of a considerable increase in CO2 concentrations. Growth under a high concentration of CO2 could stimulate carbon assimilation-especially in C3-type plants. We used a proteomics approach to test the
Paul E Abraham et al.
Nature plants, 2, 16178-16178 (2016-11-22)
Already a proven mechanism for drought resilience, crassulacean acid metabolism (CAM) is a specialized type of photosynthesis that maximizes water-use efficiency by means of an inverse (compared to C3 and C4 photosynthesis) day/night pattern of stomatal closure/opening to shift CO2

질문

  1. How many samples can be processed with this kit?

    1 답변
    1. This kit provides sufficient reagents to perform 18 chloroplast isolation procedures, using 30 grams of starting plant tissue, as described in the protocol. Please see the link below to review the product datasheet:_x000D_
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/114/229/cpisopis-ms.pdf

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