B5002

5-Bromo-2′-deoxyuridine

≥99% (HPLC)

• 동의어(들): 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR
• 실험식(Hill 표기법): C₉H₁₁BrN₂O₅
• CAS Number: 59-14-3
• Molecular Weight: 307.10
• Beilstein: 30395
• EC Number: 200-415-9
• MDL number: MFCD00006529
• UNSPSC 코드: 41106305
• PubChem Substance ID: 24278270
• NACRES: NA.51

속성

• 생물학적 소스: synthetic (organic)
• 분석: ≥99% (HPLC)
• 형태: powder
• mp: 191-194 °C (dec.) (lit.)
• solubility: DMSO: 50 mg/mL, clear, colorless to very faintly yellow
• 저장 온도: −20°C
• SMILES string: OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
• InChI: 1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1
• InChI key: WOVKYSAHUYNSMH-RRKCRQDMSA-N

설명

애플리케이션

5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.

5-Bromo-2′-deoxyuridine has been used:

• as a supplement to study the effect of electroconvulsive seizures on hippocampal neurogenesis
• as a supplement to study the loss-of-gene function in adult zebrafish heart
• in T lymphocyte proliferation assay{133]

생화학적/생리학적 작용

5-Bromo-2′-deoxyuridine (BrdU) is used to analyze cell proliferation, because of its facile incorporation into DNA during the S phase of the cell cycle. BrdU stimulates cellular differentiation and maturation in leukemia cell lines, while it inhibits differentiation of friend erythroleukemia cells. Studies of BrdU incorporation often subsequently use BrdU-specific antibodies with fluorescent tags, for detection of the incorporated BrdU, via such methods as flow cytometry or fluorescence microscopy.

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H340,H361fd
• 예방조치 성명서: P201 - P202 - P280 - P308 + P313 - P405 - P501
• Hazard Classifications: Muta. 1B - Repr. 2
• Storage Class Code: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 2
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)