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Merck
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K4002

Sigma-Aldrich

Krebs-Ringer Bicarbonate Buffer

With 1800 mg/L glucose, without calcium chloride and sodium bicarbonate, powder, suitable for cell culture

๋™์˜์–ด(๋“ค):

Bicarbonate Buffer Solution, Krebs Buffer, Krebs-Ringer Solution

๋กœ๊ทธ์ธ์กฐ์ง ๋ฐ ๊ณ„์•ฝ ๊ฐ€๊ฒฉ ๋ณด๊ธฐ

ํฌ๊ธฐ ์„ ํƒ

10 X 1 L
โ‚ฉ338,300

โ‚ฉ338,300

์˜ˆ์ƒ ์ž…๊ณ ์ผ2025๋…„ 5์›” 09์ผ์„ธ๋ถ€์‚ฌํ•ญ

๋ฒŒํฌ ๊ฒฌ์  ์š”์ฒญ
๋ชจ๋“  ์‚ฌ์ง„(1)

ํฌ๊ธฐ ์„ ํƒ

๋ณด๊ธฐ ๋ณ€๊ฒฝ
10 X 1 L
โ‚ฉ338,300

About This Item

UNSPSC ์ฝ”๋“œ:
12161702
NACRES:
NA.75

โ‚ฉ338,300

์˜ˆ์ƒ ์ž…๊ณ ์ผ2025๋…„ 5์›” 09์ผ์„ธ๋ถ€์‚ฌํ•ญ

๋ฒŒํฌ ๊ฒฌ์  ์š”์ฒญ

Quality Level

500

์–‘์‹

powder

๊ธฐ์ˆ 

cell culture | mammalian: suitable

pH

6.1-6.7

solubility

water: 9.5ย g/L, clear

์ €์žฅ ์˜จ๋„

2-8ยฐC

๊ด€๋ จ ์นดํ…Œ๊ณ ๋ฆฌ

์ผ๋ฐ˜ ์„ค๋ช…

Krebs-Ringer Bicarbonate Buffer acts as a salt solution and plays an essential role in tissue culture. A salt solution provides the cells with water and inorganic ions. It also helps in maintaining the osmotic balance and ideal pH in the medium. This bicarbonate buffered isotonic saline contains magnesium, potassium, sodium, and phosphate ions.

์• ํ”Œ๋ฆฌ์ผ€์ด์…˜

Krebs-Ringer Bicarbonate Buffer has been used:

  • as a wash buffer for the preparation of mice primary adipocytes and lipolysis assay[1]
  • in the pre-perifusion for islet viability and functionality studies[2]
  • in pituitary bioassay[3]

์ˆ˜๋Ÿ‰

Formulated to contain 9.5 grams of powder per liter of medium.

์žฌ๊ตฌ์„ฑ

Supplement with 1.26 g/L sodium bicarbonate.

๋ณด์ถฉ์ œ

์ œํ’ˆ ๋ฒˆํ˜ธ
์„ค๋ช…
๊ฐ€๊ฒฉ

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point (ยฐF)

Not applicable

Flash Point (ยฐC)

Not applicable

๊ฐ€์žฅ ์ตœ์‹  ๋ฒ„์ „ ์ค‘ ํ•˜๋‚˜๋ฅผ ์„ ํƒํ•˜์„ธ์š”:

์‹œํ—˜ ์„ฑ์ ์„œ(COA)

Lot/Batch Number
0000347442
0000347442
SLCP6729
SLCG7890
SLCB4396

์ ํ•ฉํ•œ ๋ฒ„์ „์„ ์ฐพ์„ ์ˆ˜ ์—†์œผ์‹ ๊ฐ€์š”?

ํŠน์ • ๋ฒ„์ „์ด ํ•„์š”ํ•œ ๊ฒฝ์šฐ ๋กœํŠธ ๋ฒˆํ˜ธ๋‚˜ ๋ฐฐ์น˜ ๋ฒˆํ˜ธ๋กœ ํŠน์ • ์ธ์ฆ์„œ๋ฅผ ์ฐพ์„ ์ˆ˜ ์žˆ์Šต๋‹ˆ๋‹ค.

COA ๊ฒ€์ƒ‰

์ด ์ œํ’ˆ์„ ์ด๋ฏธ ๊ฐ€์ง€๊ณ  ๊ณ„์‹ญ๋‹ˆ๊นŒ?

๋ฌธ์„œ ๋ผ์ด๋ธŒ๋Ÿฌ๋ฆฌ์—์„œ ์ตœ๊ทผ์— ๊ตฌ๋งคํ•œ ์ œํ’ˆ์— ๋Œ€ํ•œ ๋ฌธ์„œ๋ฅผ ์ฐพ์•„๋ณด์„ธ์š”.

๋ฌธ์„œ ๋ผ์ด๋ธŒ๋Ÿฌ๋ฆฌ ๋ฐฉ๋ฌธ

์ด๋ฏธ ์—ด๋žŒํ•œ ๊ณ ๊ฐ

Slide 1 of 7

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D-(+)-Glucose solution 100 g/L in H2O, sterile-filtered, BioXtra, suitable for cell culture

Sigma-Aldrich

G8644

D-(+)-Glucose solution

Sigma-Aldrich

Sigma-Aldrich

A3551

Alseverโ€ฒs Solution

Ro-20-1724 A cell-permeable, selective inhibitor of cAMP-specific phosphodiesterase (PDE IV; IC₅₀ = 2 µM).

Sigma-Aldrich

557502

Ro-20-1724

Collagenase from Clostridium histolyticum 0.2 μm filtered, suitable for release of physiologically active rat hepatocytes, Type IV-S, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Sigma-Aldrich

C1889

Collagenase from Clostridium histolyticum

Ferritin, cationized from horse spleen 10 mg/mL in 0.15 M NaCl

Sigma-Aldrich

F7879

Ferritin, cationized from horse spleen

Sodium bicarbonate solution solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

S8761

Sodium bicarbonate solution

2-Bromo-2-chloro-1,1,1-trifluoroethane ≥99%

Sigma-Aldrich

B4388

2-Bromo-2-chloro-1,1,1-trifluoroethane

Bidisha Dasgupta et al.
European journal of immunology, 38(12), 3499-3507 (2008-11-11)
When leukocytes cross endothelial cells during the inflammatory response, membrane from the recently described lateral border recycling compartment (LBRC) is selectively targeted around diapedesing leukocytes. This "targeted recycling" is critical for leukocyte transendothelial migration. Blocking homophilic PECAM interactions between leukocytes
N C Schaad et al.
Human reproduction (Oxford, England), 11(3), 561-565 (1996-03-01)
Nitric oxide is a chemical messenger which functions as a neurotransmitter or as a cytotoxic agent. Nitric oxide synthase (NOS) has been isolated from various mammalian reproductive tissues. The presence or absence of NOS in spermatozoa has not yet been
The Hypothalamic-Pituitary-Adrenal Axis Response to Stress in Mice Lacking Functional Vasopressin V1b Receptors
Stephen J L, et al.
Endocrinology, 148, 849?856-849?856 (2007)
Activation of G Protein-Coupled Receptor 43 in Adipocytes Leads to Inhibition of Lipolysis and Suppression of Plasma Free Fatty Acids
Hongfei G, et al.
Endocrinology, 149, 4519?4526-4519?4526 (2008)
Sandra E Pineda-Sanabria et al.
Journal of molecular and cellular cardiology, 92, 174-184 (2016-02-09)
One approach to improve contraction in the failing heart is the administration of calcium (Ca(2+)) sensitizers. Although it is known that levosimendan and other sensitizers bind to troponin C (cTnC), their in vivo mechanism is not fully understood. Based on
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1โ€“4 / 4 ์งˆ๋ฌธ ย 

  1. What is the Department of Transportation shipping information for this product?

    1 ๋‹ต๋ณ€

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      ๋„์›€์ด ๋˜์—ˆ์Šต๋‹ˆ๊นŒ?

  2. How should Product K4002, Krebs-Ringer Bicarbonate Buffer, be dissolved?

    1 ๋‹ต๋ณ€

    1. Product No. K4002 is listed in a 10X1L package size, meaning that each of the ten containers has enough powder to make 1 Liter of KRB buffer. It can be dissolved in 1 Liter of either water or medium.

      ๋„์›€์ด ๋˜์—ˆ์Šต๋‹ˆ๊นŒ?

  3. What is the formula of Product K4002, Krebs-Ringer Bicarbonate Buffer (KRB)?

    1 ๋‹ต๋ณ€

    1. The formulation for KRB can be found on the data sheet.

      ๋„์›€์ด ๋˜์—ˆ์Šต๋‹ˆ๊นŒ?

  4. Product K4002 - Krebs-Ringer Bicarbonate Buffer, is without calcium chloride and sodium bicarbonate. In the reconstitution steps, you advice to add 1.26g sodium bicarbonate. Should we also add some calcium chloride? in which amount?

    1 ๋‹ต๋ณ€

    1. Product K4002 - Krebs-Ringer Bicarbonate Buffer does not contain calcium. The addition of calcium is not required for osmolarity.ย  If you need to have calcium, you can add calcium chloride between 1.25 to 2.5 mM.ย  You may want to check the osmolarity and make sure it is not too high.Kreb Henseleit Buffer has 1.25 mM calcium chlorideKreb's Buffer has 2.5 mM calcium chlorideKreb' Ringer Superfusion solution contains 2.0 mM calcium chloride.Please see the attached document for different Krebs' buffer recipes.

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