추천 제품
제품명
Sodium bicarbonate solution, solution (7.5%), sterile-filtered, BioReagent, suitable for cell culture
무균
sterile-filtered
제품 라인
BioReagent
양식
solution (7.5%)
기술
cell culture | mammalian: suitable
불순물
endotoxin, tested
pH
7.8-8.2
저장 온도
2-8°C
SMILES string
[Na+].OC([O-])=O
InChI
1S/CH2O3.Na/c2-1(3)4;/h(H2,2,3,4);/q;+1/p-1
InChI key
UIIMBOGNXHQVGW-UHFFFAOYSA-M
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일반 설명
Sodium bicarbonate (NaHCo3) is a commonly used alkaline buffering agent(5) in laboratories. The addition of this buffer to the cell culture medium aids in maintaining the appropriate pH by using the carbon dioxide present in the incubator. In addition to its buffering properties, Sodium bicarbonate also acts as a source of sodium and aids in sodium gluconate synthesis during the fermentation process.
애플리케이션
Sodium bicarbonate solution has been used:
- as a component of Hanks′ buffered salt solution (HBSS) and recording medium for culturing of tissue explants from rats or mice[1]
- as a component of Dulbecco′s modified Eagle′s medium (DMEM) to culture mice tissue explants[2]
- as a component of Minimum Essential Media (MEM) to study the cytotoxicity against human colorectal adenocarcinoma cells and mammary gland adenocarcinoma[3]
- to prepare collagen mix droplets for 3D collagen sprouting assay[4]
- to prepare collagen matrix for rat epidermal keratinocyte (REK) organotypic culture[5]
제조 메모
Prepared in cell culture grade water.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
이미 열람한 고객
Phenotypic Modulation of Adipose-Derived Stem Cells and Fibroblasts Treated with Povidone--Iodine and Chlorhexidine in Mono and Coculture Models
Chelmu? BA, et al.
Biomedicines, 11(7), 1855-1855 (2023)
Nicoline Dorothea Jakobsen et al.
European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences, 178, 106282-106282 (2022-08-23)
Rat epidermal keratinocyte (REK) Organotypic culture (ROC) is an epidermis model that is robust and inexpensive to develop and maintain, and it has in previous studies been shown to have permeability characteristics close to those of human skin. Here, we
Mette Flinck et al.
Cell cycle (Georgetown, Tex.), 17(9), 1056-1067 (2018-06-14)
Precise acid-base homeostasis is essential for maintaining normal cell proliferation and growth. Conversely, dysregulated acid-base homeostasis, with increased acid extrusion and marked extracellular acidification, is an enabling feature of solid tumors, yet the mechanisms through which intra- and extracellular pH
Anna E Engler et al.
Cell reports, 33(13), 108553-108553 (2020-12-31)
There is an increasing appreciation for the heterogeneity of myeloid lineages in the lung, but relatively little is known about populations specifically associated with the conducting airways. We use single-cell RNA sequencing, flow cytometry, and immunofluorescence to characterize myeloid cells
Caroline Volz et al.
Cell reports, 31(4), 107568-107568 (2020-04-30)
Anti-angiogenic treatment targeting vascular endothelial growth factor (VEGF)-VEGFR2 signaling has shown limited efficacy in lung cancer patients. Here, we demonstrate that inhibition of VEGFR2 in tumor cells, expressed in ∼20% of non-squamous non-small cell lung cancer (NSCLC) patients, leads to
프로토콜
3-D Culture Matrix™ Collagen I facilitates cellular growth and differentiation in vitro, offering versatile applications in biomedical research.
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