The molecular weight of this product is not determined on a lot to lot basis. According to published literature, the molecular weight of Immunoglobulin G (IgG) is approximately 150 - 160 KDa. Historical data available for this product indicates that in SDS-PAGE electrophoresis this material will have multiple bands ranging from 155 kDa - 220 kDa.
추천 제품
결합
unconjugated
Grade
reagent grade
분석
≥95% (SDS-PAGE)
양식
essentially salt-free, lyophilized powder
구성
Protein, ≥90%
기술
ELISA: suitable
dot immunobinding: suitable
immunoelectrophoresis: suitable
western blot: suitable
저장 온도
2-8°C
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일반 설명
Purified Human IgG is isolated from pooled normal human serum by fractionation and ion-exchange chromatography. The product is supplied as an essentially salt-free (less than 1% sodium), lyophilized powder.
애플리케이션
IgG from human serum was used as a standard at a concentration of 1.274 mg/ml in ELISA to determine antibody responses to Porphyromonas gingivalis and Prevotella intermedia.[1] It was used for immunolabeling of Vero, BHK and PtK2 cells at a concentration of 0.2 mg/ml.[2] IgG from human serum was used as a standard in SDS-PAGE of anti-Shigella LPS antibodies.[3]
생화학적/생리학적 작용
IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.[4]
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
관련 제품
제품 번호
설명
가격
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Nils A Brechmann et al.
Biotechnology progress, 35(3), e2775-e2775 (2019-01-11)
High capacity magnetic protein A agarose beads, LOABeads PrtA, were used in the development of a new process for affinity purification of monoclonal antibodies (mAbs) from non-clarified CHO cell broth using a pilot-scale magnetic separator. The LOABeads had a maximum
Youhei Egami et al.
Journal of cell science, 130(24), 4168-4179 (2017-11-09)
Phagosome formation is a complicated process that requires spatiotemporally regulated actin reorganization. We found that RhoC GTPase is a critical regulator of FcγR-mediated phagocytosis in macrophages. Our live-cell imaging revealed that RhoC, but not RhoA, is recruited to phagocytic cups
Katinka Döhner et al.
Molecular biology of the cell, 13(8), 2795-2809 (2002-08-16)
After fusion of the viral envelope with the plasma membrane, herpes simplex virus type 1 (HSV1) capsids are transported along microtubules (MTs) from the cell periphery to the nucleus. The motor ATPase cytoplasmic dynein and its multisubunit cofactor dynactin mediate
Egil S Blix et al.
Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation, 21(5), 840-847 (2015-02-18)
Multiple myeloma (MM) is considered an incurable B cell malignancy, although many patients can benefit from high-dose therapy with autologous stem cell transplantation (ASCT) as a first-line treatment. In non-Hodgkin lymphoma (NHL), ASCT is usually performed after relapse with curative
Elisa Lázaro-Ibáñez et al.
Journal of extracellular vesicles, 8(1), 1656993-1656993 (2019-09-10)
Extracellular vesicles have the capacity to transfer lipids, proteins, and nucleic acids between cells, thereby influencing the recipient cell's phenotype. While the role of RNAs in EVs has been extensively studied, the function of DNA remains elusive. Here, we distinguished
문서
PNGase Fast denaturing buffer and enzyme provide results similar to a conventional 20-hour protocol, reducing workflow time to about 1 hour.
프로토콜
A step-by-step protocol for released N-linked glycan analysis of the monoclonal antibody adalimumab, based on UHPLC-FLR-MS and procainamide labeling.
관련 콘텐츠
Access high-quality reagents for antibody workflows, including adjuvants, blocking reagents, conjugation kits, and more.
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What is the expected MW of the product?
1 답변-
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Necesito saber la secuencia de la proteína o el número para buscarla en uniprot
1 답변-
The protein sequence is not determined for this product. Human IgG has 4 subclasses: IgG1, IgG2, IgG3 and IgG4. These are present in serum at approximately 66%, 23%, 7% and 4%, respectively.
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What is the isoelectric point of this
1 답변-
This product is not tested on a lot-to-lot basis to determine the isoelectric point. However, historical data indicates an isoelectric point range of 6.1 - 8.5.
도움이 되었습니까?
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Is product I4506, because it is derived from human serum, considered to potential biohazard?
1 답변-
As indicated in the corresponding Data Sheet at the link below: "BIOHAZARD: Handle as if capable of transmitting infectious agents". The source material is tested and found negative for antibody to HIV, antibody to HCV, and for HbsAG.
Please see the link below to review the product data sheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/100/203/i4506dat.pdf도움이 되었습니까?
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What is the significance of absorbance at 280 nm (E280 1% = 14)?
1 답변-
The absorbance at 280 nm (E280 1% = 14) indicates the percent extinction coefficient (ϵ1%).
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What is the recommended storage method for item I4506, IgG from human serum, after reconstitution?
1 답변-
After reconstitution, keep aliquots at 2-8°C for short-term use (up to 4 weeks) and store the remaining aliquots at -20°C.
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what is the expected molecular weight under reducing conditions
1 답변-
I4506, Human IgG, is a tetrameric protein consisting of 2 light chains and 2 heavy. When run on a reducing gel, The heavy chain will be detectable at ~50kDa, and the light chain at ~25kda.
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How can I use Product I4506, IgG from human serum, to block Fc receptors on the cell surface for flow cytometry analysis of cells?
1 답변-
Human cells to be used for FACS staining may be Fc-blocked by treatment with 1 μg of human IgG per 100,000 cells, for 15 minutes at room temperature. It is not necessary to wash to remove excess IgG before proceeding with staining.
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How should I store my solution of Product I4506, IgG from human serum?
1 답변-
Solutions can be stored at -20 °C for at least 1 year.
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Is Product I4506, IgG from human serum, tested for endotoxin?
1 답변-
No. Product No. I4506 is not tested for endotoxin levels.
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