The concentration of each component is listed in the Product Information Sheet. Please see the product documentation section:
https://www.sigmaaldrich.com/product/sigma/i1884#product-documentation
추천 제품
일반 설명
Insulin-transferrin-sodium selenite media supplement supports in vitro growth and maturation of follicles from oocyte and is routinely used in in vitro fertilization methods.[1]
Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine.
INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.
TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.
SELENIUM is an essential trace element normally provided by Serum.
Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.
INSULIN is a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe observed mitogenic effect to this property.
TRANSFERRIN is an iron-transport protein. Iron is an essential trace element, but can be toxic in the free form. To nourish cells in culture, it is supplied bound to transferrin in serum.
SELENIUM is an essential trace element normally provided by Serum.
Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.
애플리케이션
Insulin-transferrin-sodium selenite media supplement has been used as:
- medium 199 (M199) media supplement for cardiac myocytes culture[2]
- Roswell Park Memorial Institute (RPMI) 1640 medium component for pluripotent stem cell (iPSC) culture[3]
- Dulbecco′s Modified Eagle Medium (DMEM) supplement in ID8 ovarian carcinoma cells[4]
기타 정보
Each vial contains: ≥21 mg recombinant human insulin; ≥19 mg human transferrin (substantially iron free), and 25 μg sodium selenite.
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.
수량
Each vial sufficient to prepare 5 liters of medium.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
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시험 성적서(COA)
Lot/Batch Number
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문서
Understand how transferrin affects serum-free cell culture systems, crucial for biomanufacturing heterologous proteins like monoclonal antibodies.
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which is the concentration of each component? Insulin-transferrin-sodium selenite media supplement. I1884
1 답변-
도움이 되었습니까?
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When we add this ITS at 100X to our Ham's F12 medium it drastically adjusts the color, and the pH drops to ~6.5 from 7.2. The ITS is made up exactly as directed with 5ml acidified water and 45ml sterile water and this still occurs, why? Medium is buffered
1 답변-
The 100X stock solution of this product is prepared by reconstituting this product in 5 mL water containing 0.1 mL of Acetic acid. In general, culture media are buffered using Sodium Bicarbonate or other suitable buffers. While prepared in acidified water, the volume of the ITS stock solution added is typically insufficient to effect the pH of a buffered medium. The molarity of the acetic acid is not specified, which may be contributing to the drop in pH of the final medium. Reducing the molarity of the Acetic acid may resolve the issue, 1 M is commonly used. See the link below, at the bottom of page 1, for recommended preparation instructions. To discuss further, we kindly ask you to navigate to the link https://www.sigmaaldrich.com/techservice, click on "Report Product Issues" under the Products Section with all the required information so that a member of our team can reach out to you to assist further. Thank you.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/365/201/p6024pis.pdf
도움이 되었습니까?
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The *I1884 needs to be prepared with acidified water, which acidifies the pH of the medium when it is added to the culture medium, is it necessary to adjust the pH of the I1884 to pH 7-7.4, after preparing it and before adding it to the medium?
1 답변-
Adjusting the pH is not necessary or recommended. This is prepared at a 100X concentration. The volume added to buffered culture medium for the 1X amount is not sufficient to cause a shift in the pH.
도움이 되었습니까?
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What is the activity of insulin in I1884, Insulin-transferrin-sodium selenite media supplement (ITS Supplement)?
1 답변-
The starting material has an activity of greater than or equal to 25.2 USP units/mg insulin. The concentration of insulin at 100X is 5 mg/L.
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What is the Department of Transportation shipping information for this product?
1 답변-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
도움이 되었습니까?
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What is the stability of I1884, Insulin-transferrin-sodium selenite media supplement (ITS Supplement), in solution?
1 답변-
The stock solution can be frozen in working aliquots. Avoid repeated freeze/thaw cycles. Frozen working aliquots will be stable for at least one year. The solution is stable at 2-8°C for 30 days.
도움이 되었습니까?
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How should I reconstitute I1884, Insulin-transferrin-sodium selenite media supplement (ITS Supplement)?
1 답변-
First, make acidified water by adding acetic acid to reduce the pH to approximately 2. To prepare a stock solution, dissolve the contents of the vial in 5 mL sterile, acidified water and swirl to dissolve. Then add 45 mL of sterile water. This prepared solution is at 100X. It is diluted 100 fold (1 ml to 100 ml) to give a 1X final concentration.
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