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Merck
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문서

I2771

Sigma-Aldrich

ITS+3 Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

동의어(들):

ITS Supplement

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About This Item

UNSPSC 코드:
12352205
NACRES:
NA.75

무균

sterile-filtered

Quality Level

300

제품 라인

BioReagent

형태

liquid

기술

cell culture | mammalian: suitable

불순물

endotoxin, tested

배송 상태

ambient

저장 온도

2-8°C

일반 설명

ITS+3 liquid media supplement is a mixture of insulin, human transferrin (partially iron-saturated), sodium selenite, linoleic acid, and bovine serum albumin (BSA). It is suitable for serum-free cell culture applications.

애플리케이션

ITS+3 Liquid Media Supplement (100×) has been used as a media supplement:
  • to support the growth of human adipose stem cells (ASC)
  • to culture porcine intervertebral disc (IVD) cells and tissues
  • to culture cartilage endplate (CEP) cells

생화학적/생리학적 작용

  • Insulin: a polypeptide hormone that promotes glucose and amino acid uptake and may produce a mitogenic effect.
  • Transferrin: an iron-transport protein. Iron is an essential trace element but can be toxic in its free form. To nourish cells in culture, iron is supplied bound to transferrin in serum.
  • Selenium: an essential trace element present in serum.

성분

Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), 0.5 μg/ml sodium selenite, 470 μg/ml linoleic acid, 470 μg/ml oleic acid and 50 mg/ml bovine serum albumin at the 100x concentration. Contains 2 moles each of linoleic acid and oleic acid per mole of albumin.

제조 메모

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리 방문

Adam Cornish et al.
BMC genomics, 21(Suppl 11), 830-830 (2020-12-30)
Single-cell sequencing enables us to better understand genetic diseases, such as cancer or autoimmune disorders, which are often affected by changes in rare cells. Currently, no existing software is aimed at identifying single nucleotide variations or micro (1-50 bp) insertions and
Xue Liu et al.
iScience, 24(6), 102551-102551 (2021-06-22)
Pulmonary mesenchymal cells are critical players in both the mouse and human during lung development and disease states. They are increasingly recognized as highly heterogeneous, but there is no consensus on subpopulations or discriminative markers for each subtype. We completed
Katherine Lakstins et al.
Journal of orthopaedic research : official publication of the Orthopaedic Research Society, 39(6), 1204-1216 (2020-04-15)
Cartilage endplate degeneration/calcification has been linked to the onset and progression of intervertebral disc degeneration and there is a critical need to understand mechanisms, such as hypertrophic differentiation, of cartilage endplate degeneration/calcification to inform treatment strategies for discogenic back pain.
Di Wu et al.
International journal of stem cells, 14(1), 119-126 (2020-12-31)
The research on human hepatobiliary development and disorders has been constrained by minimal access to human fetal tissue, and low accuracy of animal models. To overcome this problem, we have established a system for the differentiation of human pluripotent stem
Hien Lau et al.
Islets, 12(3), 41-58 (2020-05-28)
The use of pancreata from pre-weaned piglets has the potential to serve as an unlimited alternative source of islets for clinical xenotransplantation. As pre-weaned porcine islets (PPIs) are immature and require prolonged culture, we developed an islet maturation media (IMM)
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문서

Linolenic Acid in Cell Culture

Importance and uses of linolenic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Selenium in Cell Culture

Understand the role of selenium in cell culture media formulations. The Media Expert library explains how culture media ingredients support cell growth.

Palmitic Acid in Cell Culture

How palmitic acid, other fatty acids and other cell culture components affect the performance of serum-free, protein-free cell culture systems used for biomanufacturing heterologous proteins including monoclonal antibodies.

Linoleic acid in Cell Culture

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

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