H9166

Vascular Endothelial Growth Factor 165 human

≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, lyophilized powder, suitable for cell culture

• 동의어(들): Endothelial Growth Factor, Vascular Growth Factor
• MDL number: MFCD00286542
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 제품명: Vascular Endothelial Growth Factor 165 human, VEGF165, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in HEK 293 cells
• 분석: ≥95% (SDS-PAGE)
• 양식: lyophilized powder
• 효능: ≤5 ng/mL EC₅₀
• 품질: endotoxin tested
• 분자량: dimer 45 kDa (glycosylated)
• 포장: pkg of 5x10 μg; pkg of 10 μg
• 제조업체/상표: HumanZyme
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1 EU/μg
• UniProt 수납 번호: P15692
• 저장 온도: −20°C
• 유전자 정보: human ... VEGFA(7422)

설명

일반 설명

Vascular endothelial growth factor 121 (VEGF121) belongs to the PDGF(platelet derived growth factor)/VEGF growth factor family.[1] The VEGFA gene is mapped to human chromosome 6p21.1.[2] The encoded protein is a dimeric glycoprotein consisting two monomers antiparallel to each other. There are five types of VEGF ligands identified in mammals, which exists as different splice variants.[3] Vascular endothelial growth factor 165 (VEGF165) belongs to the PDGF(platelet derived growth factor)/VEGF growth factor family and VEGF is secreted by many cell types.[1]HumanKine VEGF165 is expressed in human HEK 293 cells as a glycosylated homodimer with an apparent molecular mass of 45 kDa. Production in human HEK 293 cells offers authentic glycosylation. Glycosylation contributes to stability in cell growth media and other applications.

생화학적/생리학적 작용

VEGF (vascular endothelial growth factor) acts as a potent angiogenic factor and mitogen that stimulates proliferation, migration and formation of endothelial cells.[1] VEGF stimulates permeabilization of blood vessels and is present in some tumors of the nervous system.[4] VEGFA is known to interact with VEGFR co-receptors such as heparan sulphate proteoglycans and neuropilins. Mutation in the VEGFA gene results in defective vascular development. VEGF is induced by hypoxia, oncogene mutations, and cytokines such as (interleukin) IL-1, IL-8, TNF-α (tumor necrosis factor α).[3]

물리적 형태

Lyophilized from a 0.2 μm filtered solution of 1X PBS

분석 메모

The specific activity was determined by the dose-dependent stimulation of the proliferation of HUVEC cells (human umbilical vein endothelial cells).

법적 정보

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable