H5041

Cystatin C human

HumanKine^®, ≥95% (SDS-PAGE), recombinant, expressed in HEK 293 cells, suitable for cell culture

• MDL number: MFCD00163390
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 제품명: Cystatin C human, recombinant, expressed in HEK 293 cells, HumanKine^®, suitable for cell culture
• 생물학적 소스: human
• Quality Level: 100
• 재조합: expressed in HEK 293 cells
• 분석: ≥95% (SDS-PAGE)
• 양식: lyophilized powder
• 효능: ≤5 μM IC₅₀
• 품질: endotoxin tested
• 분자량: dimer 12-13 kDa (non-glycosylated)
• 포장: pkg of 10 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1 EU/mg
• UniProt 수납 번호: P01034
• 저장 온도: −20°C
• 유전자 정보: human ... CYTC(1471)

설명

일반 설명

HumanKine human Cystatin C is expressed as a non-glycosylated monomer in human HEK 293 cells. Cystatin C belongs to the cystatin superfamily.[1] Two isoforms (pI = 7.8, 9.2) of native Cystatin C are found in human urine differentiated by the elimination of small basic peptides or amino acids from the N-terminal end of the protein. This protein is coded by a housekeeping gene, CST3 located on human chromosome 20p11.21 and is generated by nucleated cells.[2]

애플리케이션

Cystatin C human has been used as a standard in western blot assay.[3] It has also been used to study its effect on the endopeptidase activity of activated Trichobilharzia regenti isoform of cathepsin B1 peptidase (TrCB1).[4]

생화학적/생리학적 작용

Cystatin C is an inhibitor of cysteine proteases including cathepsin B which has been identified as the most important β-amyloid-degrading enzyme. Measurement of cystatin C in serum is replacing creatinine as an indicator of kidney function (glomerular filtration rate, GFR). Studies show its role in predicting new-onset or deteriorating cardiovascular disease.[5][6] It also seems to play a role in brain disorders involving amyloid, such as Alzheimer′s disease.[7] Cystatin C inhibits transforming growth factor β signaling in normal and cancer cells.[1]

물리적 형태

Lyophilized from a 0.2 μm filtered solution of 1×PBS

분석 메모

The inhibitory function of cystatin c on papain′s protease activity was measured by a colorimetric assay using L-BAPA as substrate. IC₅₀ value was measured at 5 to 20 μg/mL (0.3 to 1.5 μM) with a range of 1.56 μg/mL to 50 μg/mL cystatin C in presence of 0.55 μM papain and 0.44 μM L-BAPA.

법적 정보

HumanKine is a registered trademark of Proteintech Group, Inc. and Humanzyme, Inc

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable