G8885

β-Glucuronidase from Helix pomatia

Type H-3, aqueous solution, ≥90,000 units/mL

• 동의어(들): β-D-Glucuronide glucuronosohydrolase
• CAS Number: 9001-45-0
• 효소 위원회 번호: 3.2.1.31 (BRENDA, IUBMB)
• MDL number: MFCD00130629
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 유형: Type H-3
• Quality Level: 200
• 양식: aqueous solution
• 특이 활성도: ≥90,000 units/mL
• secondary activity: ≤1,000 units/mL sulfatase
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

애플리케이션

β-Glucuronidase from Helix pomatia has been used:

• to convert isoflavone conjugates to their aglycone forms in urine and soymilk samples for high performance liquid chromatography (HPLC) detection[1]
• as a component in the protoplasting solution[2]
• for the generation of aromatic and phenolic compounds from the plasma and urine samples for gas chromatography-mass spectrometry (GC-MS)[3]
• to hydrolyze glucuronide and sulfate conjugated metabolites in fecal samples[4]

β-glucuronidase was used in the measurement of aromatization by a urine technique suitable for the evaluation of aromatase inhibitors in vivo. [5]

New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

생화학적/생리학적 작용

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

품질

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

단위 정의

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

물리적 형태

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable