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Merck
모든 사진(8)

문서

G7017

Sigma-Aldrich

β-Glucuronidase from Helix pomatia

Type HP-2, aqueous solution, ≥100,000 units/mL

동의어(들):

β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
효소 위원회 번호:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

생물학적 소스

Helix pomatia

유형

Type HP-2

형태

aqueous solution

특이 활성도

≥100,000 units/mL

secondary activity

≤7,500 units/mL sulfatase

배송 상태

wet ice

저장 온도

2-8°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

일반 설명

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the digestive juices of the Roman snail. β-glucuronidases derived from mollusks often contain sulfatase activity. Both activities are useful in the hydrolysis of metabolites for toxicology and biomarker analysis. For a broader look at our β-Glucuronidase product line and their uses see our page about Drug Metabolite Analysis

애플리케이션

  • Sample preparation for LC/MS through digestion of glucuronide conjugated compounds for biomarker and toxicology research.
  • Removal of glucuronide conjugates such as for the preparation of Baicalein.­
New Technical Article Comparing Performance of Different Enzymes
Learn more
about recent application data generated by Sigma R&D to optimize hydrolysis for different drug classes using enzymes from different sources and the use of a chromatographicaly purified enzyme to reduce the effect of esterase activity resulting in conversion of 6-MAM to Morphine

생화학적/생리학적 작용

Endogenous beta-glucuronidase is a lysosomal enzyme involved in digestion of glucuronide-modified catabolites.
Microbial beta-glucuronidase found in microbiota can cause re-absorption of metabolites by hydrolysis of glucuronide conjugates of xenobiotic and endobiotic metabolites.
Used for the hydrolysis of glucuronide conjugates in urinary metabolite analysis

특징 및 장점

  • pH optima 4.0 - 5.0.
  • Sulfatase pH optima 6.2
  • Low Sulfatase activity (<7,500 units/mL).
  • Ammonium Sulfate suspension.
  • Mollusk-derived beta-glucuronidase.
  • Useful for acidic hydrolysis of glucuronides in low pH samples.

품질

Many β-glucuronidases derived from mollusks also contain sulfatase activity.

결합

Similar to G0876, but produced by Sigma.

단위 정의

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).
Sulfatase Unit Definition: One unit of sulfatase will hydrolyze 1.0 μmole p-nitrocatechol sulfate per hr at pH 5.0 at 37 °C.

물리적 형태

Aqueous solution in ~1.0 M ammonium sulfate with 3 mM sodium azide as preservative.

픽토그램

Health hazard

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

WGK

WGK 3


시험 성적서(COA)

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문서 라이브러리 방문

W Dennis Ulrey et al.
Journal of AOAC International, 96(4), 917-924 (2013-09-05)
A multilaboratory study of AOAC Official Method 2011.23 was performed to satisfy requirements for Final Action status through the AOAC expert review panel process. The study included nine collaborating laboratories from the United States, Canada, Brazil, and The Netherlands. Five
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Journal of cardiovascular pharmacology, 47(5), 695-701 (2006-06-16)
By using red wine (RW), dealcoholized red wine (DARW), polyphenols-stripped red wine (PSRW), ethanol-water solution (ET), and water (W), the role of wine polyphenols, ethanol, and urate on vascular function was examined in humans (n = 9 per beverage) and
L Bláhová et al.
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Molecular nutrition & food research, 61(3) (2016-07-01)
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3,3'-Diindolylmethane (DIM), a major phytochemical derived from ingestion of cruciferous vegetables, is also a dietary supplement. In preclinical models, DIM is an effective cancer chemopreventive agent and has been studied in a number of clinical trials. Previous pharmacokinetic studies in

문서

β-glucuronidase (GUS) enzymes are utilized to hydrolyze glucuronide (gluc) drug metabolites to the parent drug, facilitating analysis by LC-MS/MS.

프로토콜

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

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