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Merck
모든 사진(1)

문서

G6637

Sigma-Aldrich

β-Galactose Dehydrogenase from Pseudomonas fluorescens

recombinant, expressed in E. coli, ammonium sulfate suspension, ≥50 units/mg protein (biuret)

동의어(들):

D-Galactose:NAD+ 1-oxidoreductase

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

생물학적 소스

Pseudomonas fluorescens

재조합

expressed in E. coli

분석

0.5—2.0 mg protein/mL (biuret)

형태

ammonium sulfate suspension

특이 활성도

≥50 units/mg protein (biuret)

색상

white

적합성

suitable for enzyme test

응용 분야

life science and biopharma

배송 상태

wet ice

저장 온도

2-8°C

유전자 정보

Pseudomonas fluorescens ... gdh(533113295)

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애플리케이션

β-Galactose Dehydrogenase from Pseudomonas fluorescens has been used for competitive inhibition in lectin histochemistry. It has also been used to measure the hydrolysis activity of Haloferax alicantei β-galactosidase on different disaccharides.

생화학적/생리학적 작용

β-galactose dehydrogenase catalyzes the oxidation of β-D-galactose to D-galactono-gammalactone.

단위 정의

One unit will convert 1.0 μmole of D-galactose to D-galactonate per min at pH 8.6 at 25 °C.

물리적 형태

Suspension in 3.2 M (NH4)2SO4, pH approx. 6.0

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


시험 성적서(COA)

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문서 라이브러리 방문

T E Curey et al.
Analytical biochemistry, 293(2), 178-184 (2001-06-12)
We report the development of a sensor for rapidly and simultaneously measuring multiple sugars in aqueous samples. In this strategy, enzyme-based assays are localized within an array of individually addressable sites on a micromachined silicon chip. Microspheres derivatized with monosaccharide-specific
D Peltzer et al.
Free radical research, 31 Suppl, S181-S185 (2000-02-29)
Green and white variegated leaves of Coleus blumei, Benth. were separated into albino and green sections and used to determine the distribution of vitamin C and L-galactose dehydrogenase activity, an enzyme supposed to be involved in ascorbate metabolism, in heterotrophic
C F Mazitsos et al.
European journal of biochemistry, 269(22), 5391-5405 (2002-11-09)
Protein molecular modelling and ligand docking were employed for the design of anthraquinone galactosyl-biomimetic dye ligands (galactosyl-mimodyes) for the target enzyme galactose dehydrogenase (GaDH). Using appropriate modelling methodology, a GaDH model was build based on a glucose-fructose oxidoreductase (GFO) protein
U G Jensen et al.
Clinical chemistry, 47(8), 1364-1372 (2001-07-27)
Classic galactosemia (OMIM 230400) is an inherited disorder in the metabolism of galactose caused by deficiency of the enzyme galactose 1-phosphate uridyl transferase (EC 2.7.7.12). Galactosemia leads to accumulation of galactose and galactose 1-phosphate (gal-1-P) in blood and tissues and
Sensory properties and taste compounds of fermented milk produced by Lactococcus lactis and Streptococcus thermophius
M. Omaea et al.
Food Sci. Technol., 14, 183-189 (2008)

문서

Instructions for working with enzymes supplied as ammonium sulfate suspensions

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