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Merck
모든 사진(1)

주요 문서

48275

Sigma-Aldrich

β-Galactosidase from Escherichia coli

suitable for enzyme immunoassay, lyophilized, powder, ~140 U/mg

동의어(들):

β-D-Galactoside galactohydrolase, Lactase

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About This Item

CAS Number:
효소 위원회 번호:
EC Number:
MDL number:
UNSPSC 코드:
12352204
NACRES:
NA.54

양식

powder

Quality Level

품질

lyophilized

특이 활성도

~140 U/mg

분자량

Mr ~135000

포함

phosphate buffer as stabilizer
sucrose as stabilizer

기술

enzyme immunoassay: suitable

배송 상태

wet ice

저장 온도

−20°C

유사한 제품을 찾으십니까? 방문 제품 비교 안내

애플리케이션

Aqueous solution may be used directly for derivatization without previous dialysis or gel permeation chromatography.

생화학적/생리학적 작용

β-galactosidase cleaves lactose into its monosaccharide components, glucose and galactose. It also catalyses the transglycosylation of glucose into allolactose, the inducer of β-galactosidase, in a feedback loop.

포장

Bottomless glass bottle. Contents are inside inserted fused cone.

물리적 특성

Tetramer molecular weight 465 kDa (subunits 116.3 kDa each)

단위 정의

1 U corresponds to the amount of enzyme which hydrolyzes 1 μmol 2-nitrophenyl-β-D-galactopyranoside (Cat. No. 73660) per minute at pH 7.8 and 37 °C

기타 정보

Used in a highly sensitive enzyme-linked immunoassay of human IgG with 4-methylumbelliferyl-β-D-galactoside (Catalog No. 69590); Evaluation of β-D-galactosidase (E. coli) and horse radish peroxidase as labels by sandwich enzyme immunoassay technique; Enzyme immunoassay of thyrptropin releasing hormone (TRH); Preparation of enzyme-antibody conjugates

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)


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문서 라이브러리 방문

Comparison of two methods of preparing enzyme-antibody conjugates: application of these conjugates for enzyme immunoassay.
M J O'Sullivan et al.
Analytical biochemistry, 100(1), 100-108 (1979-11-15)
M Imagawa et al.
Annals of clinical biochemistry, 21 ( Pt 4), 310-317 (1984-07-01)
beta-D-galactosidase from Escherichia coli and horseradish peroxidase were evaluated as labels of Fab' in dose-response curves for human alpha-fetoprotein and human chorionic gonadotropin by sandwich enzyme immunoassay technique using fluorogenic substrates for enzyme assay. The non-specific binding of Fab'-peroxidase conjugates
K Kato et al.
European journal of biochemistry, 62(2), 285-292 (1976-02-16)
Rabbit immunoglobulin G (IgG) was reduced by incubating with 10 mM 2-mercaptoethylamine and then treated with excess amounts of N,N'-o-phenylenedimaleimide. As a result, maleimide residues were introduced into rabbit IgG molecules. Rabbit IgG containing maleimide residues could be coupled to
M Takeyama et al.
Chemical & pharmaceutical bulletin, 40(8), 2199-2201 (1992-08-01)
A sensitive and specific double-antibody enzyme immunoassay (EIA) for a thyrotropin releasing hormone (TRH)-like immunoreactive substance has been developed. In order to synthesize TRH-labeled beta-D-galactosidase (beta-gal), a newly devised TRH derivative, pGlu-His-Pro-NH-(CH2)6-NH2 (TRH-Hex), was employed. TRH-Hex was linked to beta-gal
A Hinek et al.
The Journal of clinical investigation, 91(3), 1198-1205 (1993-03-01)
We and others have previously shown that a 67-kD cell surface elastin/laminin-binding protein (EBP) is responsible for cell adhesion to elastin and laminin and for mediating the process of elastin fiber assembly, but the nature of this protein was unknown.

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