A0168

Anti-Mouse IgG (Fc specific)–Peroxidase antibody produced in goat

affinity isolated antibody

• 동의어(들): Goat Anti-Mouse IgG (Fc specific)–HRP
• MDL number: MFCD00162629
• UNSPSC 코드: 12352203
• NACRES: NA.46

속성

• 생물학적 소스: goat
• Quality Level: 300
• 결합: peroxidase conjugate
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: secondary antibodies
• 클론: polyclonal
• 종 반응성: mouse
• 기술: direct ELISA: 1:50,000; immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100; western blot: 1:80,000-1:160,000 using total cell extract of HeLa cells (5-10 μg per well
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Antibody is isolated from goat anti-mouse IgG antiserum by immunospecific purification to remove essentially all goat serum proteins, including immunoglobulins that do not specifically bind to the Fc fragment of mouse IgG. Anti-Mouse IgG is conjugated to peroxidase by protein cross linking with 0.2% glutaraldehyde. The antibody preparation is solid phase adsorbed with human IgG to ensure minimal cross reactivity in tissue or cell preparations.

면역원

purified mouse IgG Fc fragment

애플리케이션

Anti-Mouse IgG (Fc specific)-Peroxidase antibody produced in goat has been used in

• western blot
• enzyme linked immunosorbent assay (ELISA) and
• indirect immunoperoxidase assay (IIPA)

Specificity of a new antibody for PND in blood from immunized mice was tested by Elisa using biotynlated PND and streptavidin coated plates. HRP conjugated goat anti-mouse IgG (Fc specific) was used as secondary.

물리적 형태

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT.

제조 메모

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H317
• 예방조치 성명서: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable