G4134

Glucose-6-phosphate Dehydrogenase from baker′s yeast (S. cerevisiae)

Type IX, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)

• 동의어(들): G-6-P-DH, Zwischenferment
• CAS Number: 9001-40-5
• 효소 위원회 번호: 1.1.1.49 (BRENDA, IUBMB)
• EC Number: 232-602-6
• MDL number: MFCD00081656
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 유형: Type IX
• Quality Level: 200
• 양식: lyophilized powder
• 특이 활성도: 200-400 units/mg protein (modified Warburg-Christian)
• 분자량: 128 kDa
• β-NADP 및 β-NADPH 함량: ≤10 mmol/mol
• 응용 분야: agriculture
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Research area: Cell Signaling

Glucose-6-phosphate dehydrogenase (G6PD) is a key metabolic enzyme of the pentose phosphate pathway. In S. cerevisiae, it is encoded by the ZWF1 gene. G6PD exists as a tetramer in its active form.[1]

애플리케이션

Glucose-6-phosphate dehydrogenase is used:

• To test ketose reductase activity in developing maize endosperm.[2]
• For recycling microassay of β-NADP and β-NADPH.[3][4]
• To measure the intracellular levels of NADPH and total NADP.[5]
• To measure the nicotinamide adenine dinucleotide (NAD) kinase kinetic assay activity.[6]

생화학적/생리학적 작용

Glucose-6-phosphate dehydrogenase catalyzes the conversion of glucose-6-phosphate to 6-phosphogluconolacetone as the first step in the pentose phosphate pathway.

Glucose-6-phosphate dehydrogenase catalyzes the rate-limiting step in the pentose phosphate pathway. Its function involves the conversion of glucose-6-phosphate to 6-phosphogluconolacetone while generating NADPH, which is essential for the regeneration of glutathione The glutathione system utilizes nicotinamide adenine dinucleotide phosphate hydrogen (NADPH) to effectively eliminate excess hydrogen peroxide.[7] Glucose-6-phosphate dehydrogenase (G6PD) plays an important role in regulating cell growth and survival. Their levels are higher in cells undergoing normal and neoplastic growth. Increased glucose-6-phosphate dehydrogenase activity plays a pivotal role in preventing reactive oxygen species mediated cell death.[8] Glucose-6-phosphate dehydrogenase is over expressed in several cancers whereas its activity is reduced in hyperglycemia. A deficiency in glucose-6-phosphate dehydrogenase causes hemolysis.[9]

단위 정의

One unit will oxidize 1.0 μmole of D-glucose 6-phosphate to 6-phospho-D-gluconate per min in the presence of NADP at pH 7.4 at 25 °C.

물리적 형태

Lyophilized powder essentially sulfate-free, containing approx. 20% sodium citrate

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)