G3776

Guanosine 5′-triphosphate sodium salt solution

HPLC purified, aqueous solution for RNA polymerase transcription

• 동의어(들): GTP
• CAS Number: 36051-31-7
• MDL number: MFCD00077781
• UNSPSC 코드: 41106305
• PubChem Substance ID: 24895138
• NACRES: NA.52

속성

• 생물학적 소스: Porcine brain; bacterial (Corynebacterium); yeast
• Quality Level: 300
• 분석: ≥95% (HPLC)
• 양식: liquid
• 농도: 100 mM in H₂O (adjusted to pH 7)
• 색상: colorless
• 외래 활성: DNase, RNase and Nickase, none detected
• 배송 상태: dry ice
• 저장 온도: −20°C
• SMILES string: [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]3O)C(=O)N1
• InChI: 1S/C10H16N5O14P3.3Na/c11-10-13-7-4(8(18)14-10)12-2-15(7)9-6(17)5(16)3(27-9)1-26-31(22,23)29-32(24,25)28-30(19,20)21;;;/h2-3,5-6,9,16-17H,1H2,(H,22,23)(H,24,25)(H2,19,20,21)(H3,11,13,14,18);;;/q;3*+1/p-3/t3-,5-,6-,9-;;;/m1.../s1
• InChI key: KZRMTEVIDYXWQW-CYCLDIHTSA-K
• 유전자 정보: human ... HRAS(3265)

설명

애플리케이션

Guanosine 5′-triphosphate sodium salt solution has been used:

• for reconstituting transducin during purification of transducin.[1]
• as a compnent of the 3.3X loading buffer to assemble 32P-miR-31—3xFLAG-AGO2 complexes[2]
• as a component of the nucleotide substrate mix to evaluate the hydrolytic activity of MutT homolog 1 (MTH1) on methylated nucleoside triphosphates[3]
• in DNA-dependent RNA polymerase transcription

생화학적/생리학적 작용

Guanosine 5′-triphosphate (GTP) forms nearly 25% of the total nucleotide triphosphate pool and is a nucleotide anion.[4] It participates in several biological functions such as cell signaling by activation of GTP-binding proteins, RNA and protein synthesis[4], and production of cyclic guanosine monophosphate (cGMP), a secondary messenger. GTP also participates in the production of stringent alarmone.[4]

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type N95 (US)