콘텐츠로 건너뛰기
Merck
모든 사진(2)

주요 문서

모든 문서 보기

A6559

Sigma-Aldrich

Adenosine 5′-triphosphate disodium salt solution

Crystalline ATP, HPLC purified, aqueous solution for RNA transcription

동의어(들):

ATP

로그인조직 및 계약 가격 보기

크기 선택

25 μMOL
₩225,229

₩225,229

출고 가능일2025년 4월 01일세부사항

모든 사진(2)

크기 선택

보기 변경
25 μMOL
₩225,229

About This Item

CAS Number:
987-65-5
MDL number:
MFCD00080339
UNSPSC 코드:
41106305
eCl@ss:
32160414
PubChem Substance ID:
24891106
NACRES:
NA.52

₩225,229

출고 가능일2025년 4월 01일세부사항

Quality Level

300

양식

liquid

농도

100 mM in H2O (adjusted to pH 7 with Trizma®
base)

외래 활성

DNase, RNase, none detected

배송 상태

dry ice

저장 온도

−20°C

SMILES string

[Na+].[Na+].Nc1ncnc2n(cnc12)[C@@H]3O[C@H](COP(O)(=O)OP([O-])(=O)OP(O)([O-])=O)[C@@H](O)[C@H]3O

InChI

1S/C10H16N5O13P3.2Na/c11-8-5-9(13-2-12-8)15(3-14-5)10-7(17)6(16)4(26-10)1-25-30(21,22)28-31(23,24)27-29(18,19)20;;/h2-4,6-7,10,16-17H,1H2,(H,21,22)(H,23,24)(H2,11,12,13)(H2,18,19,20);;/q;2*+1/p-2/t4-,6-,7-,10-;;/m1../s1

InChI key

TTWYZDPBDWHJOR-IDIVVRGQSA-L

유사한 제품을 찾으십니까? 방문 제품 비교 안내

관련 카테고리

일반 설명

Adenosine 5′-triphosphate disodium salt solution is suitable for use in molecular biology applications such as kinase reactions or DNA-dependent RNA polymerase transcription.

애플리케이션

Adenosine 5′-triphosphate disodium salt solution has been used:
  • as a standard to evaluate the extracellular ATP release from marginal cells of stria vascular[1]
  • as a component of the internal pipette solution to establish a recording electrode to study neurite stretching[2]
  • in in vitro VPS34 (catalytic subunit of the phosphatidylinositol 3-kinase (PtdIns3K) complex) activity assay[3]
  • as a component of lysis and resealing buffers for ATP studies[4]
  • in spectrophotometric measurement of H+ transport in fluorescein isothiocyanate (FITC)-dextran loaded A549 (adenocarcinomic human alveolar basal epithelial cells) and HeLa cells[5]
  • to induce pyroptosis in lipopolysaccharide treated fibroblast-like synoviocytes (FLSs)[6]

생화학적/생리학적 작용

Adenosine 5′-triphosphate (ATP) is an important energy currency in all living organisms. It possesses high phosphate transfer potential. ATP is involved in several biological processes such as membrane transport, muscle contraction and synthesis, and degradation of biological molecules. It plays a role as an intracellular and extracellular signaling molecule in certain cellular processes such as cell motility, organ development, neurotransmission, and insulin secretion.[7]

법적 정보

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

가장 최신 버전 중 하나를 선택하세요:

시험 성적서(COA)

Lot/Batch Number
0000394151
0000366958
0000394151
0000366958
0000274502

적합한 버전을 찾을 수 없으신가요?

특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

이 제품을 이미 가지고 계십니까?

문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Yahui Huang et al.
Archiv der Pharmazie (2018-06-29)
Inhibition of spleen tyrosine kinase (Syk) is a promising strategy for the treatment of various allergic and autoimmune disorders such as asthma, rheumatoid arthritis, and allergic rhinitis. Previously, a Syk inhibitor with novel indazole scaffold was discovered by structure-based virtual
James Franklin Tucker et al.
PLoS genetics, 12(2), e1005873-e1005873 (2016-02-20)
Epigenetic gene silencing plays a critical role in regulating gene expression and contributes to organismal development and cell fate acquisition in eukaryotes. In fission yeast, Schizosaccharomyces pombe, heterochromatin-associated gene silencing is known to be mediated by RNA processing pathways including
Cooper GM
The Cell: A Molecular Approach (2000)
Identification of small molecule inhibitors of phosphatidylinositol 3-kinase and autophagy.
Farkas T
The Journal of Biological Chemistry, 286(45), 38904-38912 (2011)
Shaojian Chen et al.
Cell cycle (Georgetown, Tex.), 20(12), 1107-1121 (2021-06-08)
Studies have found that cell pyroptosis is involved in the occurrence and development of rheumatoid arthritis (RA). Hsa_circ_0044235 has been found to be significantly low-expressed in RA patients. The purpose of this research was to reveal the regulatory mechanism of
모든 관련된 서류 보기

문서

mRNA Synthesis for the Development of Vaccines and Therapeutics

Understand how mRNA vaccines induce immunity. and read how synthetic mRNA is prepared for vaccine immunogens and other biopharmaceuticals. Find reagents for synthesis of mRNA.

질문

1–9 / 9 질문  

  1. How does the storage temperature relate to shipping conditions?

    1 답변

    1. The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment.

      도움이 되었습니까?

  2. Can this preparation of Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used as an ATP standard?

    1 답변

    1. This preparation of ATP is not recommended for use as an ATP standard. The recommended ATP products for use as a standard in bioluminescence experiments is Product Code FL-AAS (preweighed vials of 1 mg each = 2 micromole).

      도움이 되었습니까?

  3. What is the binding affinity of ATP (Product No. A6559) for Mg++ and other metal ions?

    1 답변

    1. ATP has intrinsic metal binding affinity. The binding constant for various metals are (given as per mole): Mg++, 9554; Na+, 13; Ca++, 3722; K+, 8; Sr++, 1381; Li+, 25.  The ability of ATP to chelate magnesium results in effective Mg++ ion concentrations lower than the total Mg ion concentration.  For example, in a reaction with 1.5mM Mg++ and 0.2 mM ATP, the effective Mg ion concentration is ~1.3mM.

      도움이 되었습니까?

  4. Can Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used in molecular biology applications?

    1 답변

    1. This is a Molecular Biology grade ATP. It is certified free of DNase and RNase. This product is recommended for any typical molecular biology application.

      도움이 되었습니까?

  5. Can Product A6559, Adenosine 5´-triphosphate disodium salt solution, be used for in vitro transcription?

    1 답변

    1. The purity of this product should be sufficient for use in T7 RNA polymerase-catalyzed in vitro transcription assay, but we do not test the product for use in that application in our quality control process.

      도움이 되었습니까?

  6. How long can aqueous ATP solutions like Product No. A6559 be stored?

    1 답변

    1. Aqueous solutions of ATP are stable for months when frozen in solution at -15 °C and for approximately one week at 0 °C.  As with most products, we guarantee the quality of this material for 1 year when stored unopened at the recommended storage temperature (-20°C in this case).

      도움이 되었습니까?

  7. What is the millimolar extinction coefficient for ATP, Product No. A6559?

    1 답변

    1. The millimolar extinction coefficient (EmM) is 15.4, measured at 259 nm for a sample in 100 mM phosphate buffer, pH 7.0.

      도움이 되었습니까?

  8. What is meant by 25UMO in the size description of Product A6559, Adenosine 5´-triphosphate disodium salt solution?  What is the product dissolved in?

    1 답변

    1. 25UMO means 25 micromoles.  The 25 UMO size is equivalent to 250 μl of a 100 mM solution. This product is a 100 mM solution in water; the solution pH has been adjusted with Trizma base but contains no additional buffer or salts.

      도움이 되었습니까?

  9. What is the Department of Transportation shipping information for this product?

    1 답변

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      도움이 되었습니까?

후기

평점 값 없음

활성 필터

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

고객지원팀으로 연락바랍니다.