FLE250

Firefly Lantern Extract

for ATP determination

• 동의어(들): FLE
• UNSPSC 코드: 12352202
• NACRES: NA.81

속성

• Quality Level: 200
• 양식: solution
• 포장: pkg of 250 mg dried lanterns
• 기술: toxicology assay: suitable
• 저장 온도: −20°C

설명

일반 설명

The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract.[1] The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure.[2] The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.[1]

애플리케이션

Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.

Firefly Lantern Extract has been used in the detection of ATP concentrations by bioluminescence flash analysis in seawater samples,[3] bioluminescence assay in river sediments samples,[4] marine sediments[5] and in quantifying extracellular ATP in red blood cells.[6]

생화학적/생리학적 작용

Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg²⁺ to yield a light-emitter oxyluciferin.[2]

기타 정보

Crude luciferin and luciferase

재구성

After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO₄^.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable