F5392

Fibroblast Growth Factor-Basic from bovine pituitary

~90% (SDS-PAGE and N-terminal analysis), lyophilized powder, suitable for cell culture

• 동의어(들): FGF2, bFGF
• CAS Number: 106096-93-9
• MDL number: MFCD00081382
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• 제품명: Fibroblast Growth Factor-Basic from bovine pituitary, FGF-Basic, suitable for cell culture
• 생물학적 소스: bovine pituitary glands
• Quality Level: 200
• 분석: ~90% (SDS-PAGE and N-terminal analysis)
• 양식: lyophilized powder
• 효능: 0.03-3.0 ng/mL ED₅₀/EC₅₀
• 품질: endotoxin tested
• 분자량: 16.4 kDa
• 포장: pkg of 1 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: <0.5 EU/vial
• 색상: white
• UniProt 수납 번호: P03969
• 저장 온도: −20°C
• 유전자 정보: bovine ... FGF2(281161)

설명

일반 설명

The fibroblast growth factor-2 (FGF2) gene is predominantly expressed in uterus, cornea and vascular endothelial cells.[1] The FGF2 gene is mapped to cattle chromosome 17.[2]

애플리케이션

A natural mitogenic growth factor used in cell culture applications to study cell signaling and to promote fibroblast and endothelial cell growth and survival.
Fibroblast Growth Factor-Basic from bovine pituitary has been used:

• In animal and human cell culture[3]
• In neuronal induction of adipose and bone marrow-derived rat mesenchymal stem cells[4]
• In the generation of directly reprogramming fibroblast[5]
• In human glioma cell culture[6]

생화학적/생리학적 작용

Fibroblast growth factor-2 (FGF2) stimulates cell proliferation, differentiation and migration. It is also known to participate in angiogenesis and carcinogenesis. During the estrous cycle, FGF2 expression is found to be maximum in porcine conceptuses and endometrium. Upregulation of this gene is also observed in pregnancy during peri-implantation. FGF2 is involved in embryogenesis, tissue repair and other metabolic pathways.[1]

물리적 형태

Lyophilized from a 0.2 μm filtered solution in sodium phosphate and sodium chloride containing 0.1 mg bovine serum albumin

제조 메모

Purified glands by a modification of the method of Gospodarowicz, et al.[7]

분석 메모

The proliferative activity is tested in culture using fetal bovine heart endothelial cells.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)