D2916

Lambda DNA Mixed Digest

for DNA electrophoresis

• UNSPSC 코드: 12352200
• NACRES: NA.25

속성

• 양식: buffered aqueous solution
• 사용: 100 uses (0.1 mg size); 20 uses (20 μg size)
• 적합성: suitable for electrophoresis (DNA)
• 저장 온도: −20°C

설명

일반 설명

Sigma′s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.

애플리케이션

Lambda DNA Mixed Digest has been used for DNA electrophoresis.[1]

Suitable for size determination of dsDNA by electrophoresis with either agarose or polyacrylamide gels.

성분

Sigma′s Lambda DNA Mixed Digest is provided in a solution of 10 mM Tris-HCl (pH 8.0), with 1.0 mM EDTA.

제조 메모

Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.

기타 정보

For optimal resolution, the recommended agarose gel concentration is 0.4%.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable