S9430

SYBR^® Green I nucleic acid gel stain

10,000 × in DMSO

• 동의어(들): DNA stain, SYBR^® green gel dye, safer gel stain
• CAS Number: 163795-75-3
• MDL number: MFCD00284715
• UNSPSC 코드: 12171500
• NACRES: NA.52

속성

• 형태: solution
• Quality Level: 200
• 사용: 1.0 mL sufficient for 100 mini-gels
• 환경친화적 대안 제품 특성: Designing Safer Chemicals; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• 농도: 10,000 × in DMSO
• 기술: PCR: suitable
• 환경친화적 대안 카테고리: Aligned,
• 저장 온도: −20°C

설명

일반 설명

SYBR^® Green I is a proprietary asymmetrical cyanine dye, which is used to detect nucleic acids. It consists of a N-alkylated benzothiazolium or benzoxazolium ring system, that is joined by a monomethine bridge to a pyridinium or quinolinium ring system. SYBR Green I binds to the minor groove of dsDNA and is excited at a wavelength of 480 nm. It has a peak fluorescence emission of 520 nm.

애플리케이션

SYBR^® Green I nucleic acid gel stain has been used for:

• the quantification of dsDNA
• to stain DNA in polymerase chain reaction (PCR)
• for comet assay technique
• to assess spermatozoon membrane integrity
• for visual inspection of DNA amplified by loop-mediated isothermal amplification (LAMP)
• as a fluorescent dye in flow cytometry
• real-time quantitative reverse transcription-polymerase chain reaction (qRT-PCR) for staining reverse-transcribed cDNA

특징 및 장점

• An ultrasensitive stain for post-electrophoresis staining of dsDNA in agarose or polyacrylamide gels
• It can also detect ssDNA and RNA in denaturing agarose/formaldehyde and polyacrylamide/urea gels without any pre-washing steps
• It is less mutagenic than ethidium bromide in Ames tests
• It provides 50-100 times greater detection sensitivity than ethidium bromide for oligonucleotides
• Useful for many applications with a limited amount of DNA
• The binding of SYBR^® Green I to DNA does not inhibit the activity of many common restriction endonucleases, including Hind III and EcoR I
• Removal of this stain in-gel digestion and ligation techniques is not needed
• SYBR Green I is a greener alternative product to ethidium bromide for staining

저장 및 안정성

Store the product at –20 °C. The diluted Staining Solution may be stored, protected from light, either at 2–8 °C for several weeks or at room temperature for 3–4 days.

법적 정보

SYBR is a registered trademark of Life Technologies

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): 201.2 °F - closed cup
• Flash Point (°C): 94 °C - closed cup
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)