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Merck
모든 사진(2)

주요 문서

CWA-1080

Sigma-Aldrich

Anti-Human CD178/FasL (NOK-1) ColorWheel® Dye-Ready mAb

greener alternative

for use with ColorWheel® Dyes (Required, (Sold Separately))

동의어(들):

Tumor necrosis factor ligand superfamily member 6

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About This Item

UNSPSC 코드:
12352203
NACRES:
NA.41
제품 CWA-1080은(는)귀하의 국가에서 현재 판매되지 않습니다. 고객지원팀으로 연락바랍니다.

생물학적 소스

mouse

Quality Level

항체 형태

purified antibody

항체 생산 유형

primary antibodies

클론

NOK-1, monoclonal

제품 라인

ColorWheel®

양식

lyophilized

분자량

calculated mol wt 31.49 kDa

정제법

using Protein A

종 반응성

human

포장

antibody small pack of 25 μL

환경친화적 대안 제품 특성

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

기술

flow cytometry: suitable

동형

IgG1κ

에피토프 서열

C-terminal

단백질 ID 수납 번호

UniProt 수납 번호

호환성

for use with ColorWheel® Dyes (Required, (Sold Separately))

환경친화적 대안 카테고리

배송 상태

ambient

저장 온도

2-8°C

타겟 번역 후 변형

unmodified

유전자 정보

human ... FASLG(356)

일반 설명

ColorWheel® technology is a novel and proprietary method of creating your own antibody and dye combinations for use in flow cytometry. By incubating any ColorWheel® antibody with any ColorWheel® dye, researchers can quickly and simply produce primary conjugated antibodies for use in single color or multicolor flow cytometry analysis. Each ColorWheel® antibody and dye is lyophilized for long long-term storage, allowing for simplicity and flexibility without compromise. ColorWheel® technology requires both ColorWheel® antibody and ColorWheel® dye for Flow Cytometry application.
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This product is Preservative-free, lyophilized product for enhanced stability and allow for ambient shipping and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

특이성

Clone NOK-1 is a mouse monoclonal antibody that detects CD178 (FasL).

면역원

L5178Y mouse T lymphoma cells expressing recombinant human CD178.

애플리케이션

Quality Control Testing

Evaluated by Flow Cytometry in HEK293 cells transfected with CD178.

Staining of one million transiently CD178-transfected HEK 293 cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1080, Anti-Human CD178/FasL (NOK-1) ColorWheel® Dye-Ready mAb and Cat. No. CWDS-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye or a 1:1 mixture of an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWDS-APC ColorWheel® Antibody-ready Allophycocyanin (APC) Dye.

Evaluated by Flow Cytometry in NK cells.

Flow Cytometry Analysis: Staining of one million Human NK cells was performed using 5 μL of a 1:1 mixture of Cat. No. CWA-1080, Anti-Human CD178 (NOK-1) ColorWheel® Dye-Ready mAb and Cat. No. CWD-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye or an equivalent amount of Cat. No. CWA-1013 Mouse IgG1 isotype control and Cat. No. CWD-APC ColorWheel® Antibody-Ready Allophycocyanin (APC) Dye.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

호환성

Requires ColorWheel® Dye (Sold Separately)

표적 설명

Tumor necrosis factor ligand superfamily member 6 (UniProt: P48023; also known as Apoptosis antigen ligand, APTL, CD95 ligand, CD95-L, Fas antigen ligand, Fas ligand, FasL, CD178) is encoded by the FASLG (also known as APT1LG1, CD95L, FASL, TNFSF6) gene (Gene ID: 356) in human. Fas Ligand is a single-pass, homotrimeric, type II membrane protein with a cytoplasmic domain (aa 1-80), a transmembrane domain (aa 81-102), and an extracellular domain (aa 103-281). The membrane-bound FasL (mFasL) undergoes two successive intramembrane proteolytic cleavages. The first one is processed by ADAM10, which produces an N-terminal fragment that lacks the receptor-binding extracellular domain. This ADAM10-processed FasL remnant form is still membrane anchored and further processed by signal peptide peptidase like 2A (SPPL2A) that liberates the FasL intracellular domain (FasL ICD). mFasL can also undergo proteolytic cleavage to produce a truncated soluble form (sFasL; aa 130-281). However, sFasL does not activate Fas, and it competes with the membrane bound FasL to reduce its cytotoxic activity. sFasL can bind to Fas, but does not induce its oligomerization, thereby preventing activation of the proapoptotic signaling pathway by the membrane bound FasL. FasL can also undergo N-glycosylation that enhances its apoptotic activity. Two isoforms of FasL have been described that are produced by alternative splicing. (Ref.: Volpe, E., et al. (2016). Front. Immunol. 7; 382; Schneider, P., et al. (1997). J. Biol. Chem. 272(30); 18827-18833).

물리적 형태

Lyophilized from PBS containing D-Mannitol and Sucrose, normal appearance is a dried pellet. Reconstituted antibody solution is stable and functional as assessed by functional testing. Contains no biocide or preservatives, such as azide.

재구성

1.0 mg/mL after reconstitution at 25 μL in PBS. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

저장 및 안정성

Recommend storage of lyophilized product at 2-8°C. Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial. Reconstitute each vial by adding 25 μL PBS. Protect from light.

법적 정보

ColorWheel is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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제품 번호
설명
가격

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

Lot/Batch Number

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문서 라이브러리 방문

관련 콘텐츠

ColorWheel® 유세포 분석 항체와 염료를 통해 유세포 분석 설계에 자유를 누리십시오. ColorWheel® 유세포 분석 포트폴리오는 품질의 저하 없이 유세포 분석 워크플로를 간소화할 수 있도록 단순화와 유연성을 염두에 두고 설계되었습니다.

질문

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활성 필터

자사의 과학자팀은 생명 과학, 재료 과학, 화학 합성, 크로마토그래피, 분석 및 기타 많은 영역을 포함한 모든 과학 분야에 경험이 있습니다..

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