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Merck
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주요 문서

CS0760

Sigma-Aldrich

Isolated Mitochondria Staining Kit

1 kit sufficient for 50 reactions (in a 2 mL cuvette), 1 kit sufficient for 1000 reactions (using 96 multiwell plates)

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크기 선택

1 KIT
₩867,927

₩867,927


구입 가능 여부는 고객센터에 문의하십시오.


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1 KIT
₩867,927

About This Item

EC Number:
UNSPSC 코드:
12352207
NACRES:
NA.32

₩867,927


구입 가능 여부는 고객센터에 문의하십시오.

Quality Level

사용

 kit sufficient for 1000 reactions (using 96 multiwell plates)
 kit sufficient for 50 reactions (in a 2 mL cuvette)

포장

pkg of 1 kit

저장 조건

dry at room temperature

기술

flow cytometry: suitable
protein staining: suitable

형광

λex 490 nm; λem 590 nm (JC-1 dye)

응용 분야

cell analysis
detection

검출 방법

fluorometric

배송 상태

dry ice

저장 온도

−20°C

애플리케이션

Isolated Mitochondria Staining Kit has been used to assess mitochondrial inner membrane potential.[1][2][3]

특징 및 장점

  • Fast, simple, and convenient method for staining isolated mitochondria and for assaying mitochondria intactness
  • Contains all the reagents required for the detection of changes in mitochondrial inner-membrane electrochemical potential
  • Contains the antibiotic Valinomycin that permeabilizes the mitochondrial membrane, and thus, dissipates the mitochondrial electrochemical potential. Valinomycin can be used as a control agent that prevents JC-1 aggregation

원리

The kit is based on mitochondria staining using the JC-1 dye. In normal cells, the JC-1 dye concentrates in the mitochondrial matrix where it forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential (e.g. apoptosis) does not allow the accumulation of JC-1 dye in the mitochondria. The dye is detected using a fluorimeter or fluorescence microscope.

분석 메모

The kit measures mitochondria intactness and detects events that dissipate the mitochondrial membrane potential.

키트 구성품 전용

제품 번호
설명

  • DMSO 1 mL

  • JC-1 Assay Buffer 5X 25 mL

  • JC-1 Stain 25 μg

  • Valinomycin Ready Made 100 μL

픽토그램

Skull and crossbones

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Oral

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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시험 성적서(COA)

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Neuroprotective efficacy of mitochondrial antioxidant MitoQ in suppressing peroxynitrite-mediated mitochondrial dysfunction inflicted by lead toxicity in the rat brain
Maiti AK, et al.
Neurotoxicity Research, 31(3), 358-372 (2017)
Arpan Kumar Maiti et al.
Interdisciplinary toxicology, 11(4), 306-315 (2019-11-26)
Nickel is a potential neurotoxic pollutant inflicting damage in living organisms, including fish, mainly through oxidative stress. Previous studies have demonstrated the impact of nickel toxicity on mitochondrial function, but there remain lacunae on the damage inflicted at mitochondrial respiratory
Subcellular fractionation of mitochondria.
Rice, J.E., and Lindsay, J.G. et al.
Subcellular Fractionation, A Practical Approach, 107- 142 (1997)
Joanna Skalska et al.
Neurotoxicity research, 38(3), 650-664 (2020-06-27)
Extensive incorporation of silver nanoparticles (AgNPs) into many medical and consumer products has raised concerns about biosafety. Since nanosilver accumulates persistently in the central nervous system, it is important to assess its neurotoxic impacts. We investigated a model of prolonged
Differential expression of choline kinase isoforms in skeletal muscle explains the phenotypic variability in the rostrocaudal muscular dystrophy mouse
Wu G, et al.
Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids, 1801(4), 446-454 (2010)

문서

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.

질문

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