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Merck
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70584-M

Millipore

BugBuster® Protein Extraction Reagent

동의어(들):

Protein Extraction Reagent, Protein Extraction Solution

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크기 선택

500 ML
₩686,445

₩686,445

예상 입고일2025년 1월 31일세부사항

벌크 견적 요청
모든 사진(1)

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보기 변경
500 ML
₩686,445

About This Item

UNSPSC 코드:
41106516
NACRES:
NA.32

₩686,445

예상 입고일2025년 1월 31일세부사항

벌크 견적 요청

양식

liquid

Quality Level

100

제조업체/상표

Novagen®

저장 조건

OK to freeze
avoid repeated freeze/thaw cycles

저장 온도

10-30°C

일반 설명

BugBuster® Protein Extraction Reagent is formulated for gentle disruption of Escherichia coli cell wall to liberate active proteins. It provides a simple, rapid, low-cost alternative to mechanical methods such as French Press or sonication for releasing expressed target protein in preparation for purification or other applications. The proprietary formulation utilizes a tris-buffer-based mixture of non-ionic and zwitterionic detergents that is capable of cell wall perforation without denaturing the protein. BugBuster is also useful for the preparation of high-purity inclusion bodies for subsequent purification in instances where expressed proteins are insoluble.

애플리케이션

BugBuster® Protein Extraction Reagent has been used:
  • to lyse Escherichia coli BLR (DE3) cells for β-glucosidase (BglB) production and purification[1]
  • as a cell lysis buffer to treat the synechocystis PCC6803 transconjugants and measure the yellow fluorescent protein (YFP) fluorescence[2]
  • in lysis buffer for purification of glutathione S-transferase- heat shock protein family A (Hsp70) member 8 (GST- HSPA8)[3]

경고

Toxicity: Standard Handling (A)

법적 정보

BUGBUSTER is a registered trademark of Merck KGaA, Darmstadt, Germany
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

시험 성적서(COA)

제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Patricia Armshaw et al.
Biotechnology for biofuels, 8, 201-201 (2015-12-03)
The use of photosynthetic autotrophs and in particular the model organism Synechocystis PCC6803 is receiving much attention for the production of sustainable biofuels and other economically useful products through metabolic engineering. Optimisation of metabolic-engineered organisms for high-level sustained production of
Dylan Alexander Carlin et al.
PloS one, 12(5), e0176255-e0176255 (2017-05-23)
Accurate modeling of enzyme activity and stability is an important goal of the protein engineering community. However, studies seeking to evaluate current progress are limited by small data sets of quantitative kinetic constants and thermal stability measurements. Here, we report
Tiejian Nie et al.
Autophagy, 17(10), 2923-2938 (2021-05-11)
As a highly dynamic organelle, mitochondria undergo constant fission and fusion to change their morphology and function, coping with various stress conditions. Loss of the balance between fission and fusion leads to impaired mitochondria function, which plays a critical role

문서

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

Citation Spotlight: BugBuster® Protein Extraction Reagent for Efficient Protein Extraction from Bacterial Pathogens

BugBuster® and Benzonase® Reagents are the Clear Solutions to Simple, Efficient Extraction of E. coli Proteins

This article shows the use of BugBuster® and Benzonase® as protein purification tools to extract recombinant proteins from E. coli and to reduce the viscosity of the extract.

ReadyShield Phosphatase and Protease Inhibitor Cocktail FAQ

ReadyShield® phosphatase and protease inhibitor cocktail FAQ for sample protection in a variety of cell types and tissue extracts, including mammalian, plant, and microbial samples. Our ReadyShield® Protease Inhibitor Cocktail is a non-freezing solution that contains inhibitors with a broad specificity for serine, cysteine, acid proteases and aminopeptidases.

관련 콘텐츠

용해 및 단백질 추출

세제 용해화, 냉동-해동 용해, 삼투압 충격, 음파파쇄, 효소성 세포 용해 및 Dounce, Polytron 그리고 절구와 절구공이 균질화와 같은 기계적 파쇄법을 포함한 세포 용해와 단백질 추출법의 개요.

Lysis & Protein Extraction

Cell lysis and protein extraction methods overview various techniques, from detergent solubilization to mechanical disruption, supporting research needs.

질문

1–3 / 3 질문  

  1. Can this be used to extract membrane proteins?

    1 답변

    1. BugBuster Protein Extraction Reagent is a mixture of non-ionic and zwitterionic detergents optimized for the extraction of soluble proteins. It does not solubilize insoluble proteins in inclusion bodies. BugBuster does not lyse cells but creates openings in the membrane to release proteins, similar to most detergent-based extractions. Traditionally, detergents are used to release membrane proteins into the soluble fraction, although not all membrane proteins will be released. Some may remain in the pellet and require denaturing conditions for release. Highly hydrophobic or membrane-associating proteins are likely to be found in the cell debris pellet. The extraction of membrane proteins using BugBuster has not been directly evaluated. Non-ionic detergents can solubilize some membrane proteins, but the extent to which BugBuster achieves this has not been analyzed. It is expected that some, but not all, membrane proteins would be solubilized using BugBuster, and therefore, its use for membrane protein extraction cannot be guaranteed.

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  2. can we use this for protein extarction of P aeruginosa bacteria for Carb NP test

    1 답변

    1. BugBuster® Protein Extraction Reagent is formulated for gentle disruption of E. coli cell wall. It is not tested for use with other organisms. Suitability would have to be determined experimentally.

      도움이 되었습니까?

  3. Can I use bugbuster to lysis Mycobacterium tuberculosis(ATCC25177)?

    1 답변

    1. BugBuster® Protein Extraction Reagent is formulated for gentle disruption of E. coli cell wall. It is not tested for use with other organisms. Suitability would have to be determined experimentally.

      도움이 되었습니까?

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