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Merck
모든 사진(6)

주요 문서

CMC0018

Sigma-Aldrich

OverExpress™ C41(DE3) pLysS Chemically Competent Cells

Escherichia coli, rod shaped

동의어(들):

Chemically competent cells

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모든 사진(6)

About This Item

UNSPSC 코드:
41106202
NACRES:
NA.85

제품명

OverExpress™ C41(DE3) pLysS Chemically Competent Cells, for the highest protein expression

생물학적 소스

Escherichia coli

Grade

Molecular Biology

성장 모드

adherent or suspension

형태학

rod shaped

기술

microbiological culture: suitable

세포 형질전환

competent cell type: chemically competent
transformation efficiency: ≥1 x 106 cfu/μg

배송 상태

dry ice

저장 온도

−70°C

일반 설명

OverExpress™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3) pLysS (CmR)

특징 및 장점

  • Express genes cloned into any T7 vector with these BL21(DE3) derivatives
  • Effective in expressing toxic & membrane proteins
  • Cited in over 350 research articles

성분

  • OverExpress C41(DE3) pLysS chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for expression


법적 정보

OverExpress is a trademark of Imaxio, S.A.

Storage Class Code

10 - Combustible liquids

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시험 성적서(COA)

Lot/Batch Number
SLCN4477
SLCM4782
SLCK1974
SLCF6527
SLCD2472

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특정 버전이 필요한 경우 로트 번호나 배치 번호로 특정 인증서를 찾을 수 있습니다.

COA 검색

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Ji-Young Youn et al.
Molecular biology of the cell, 21(17), 3054-3069 (2010-07-09)
BAR domains are protein modules that bind to membranes and promote membrane curvature. One type of BAR domain, the N-BAR domain, contains an additional N-terminal amphipathic helix, which contributes to membrane-binding and bending activities. The only known N-BAR-domain proteins in
Myles B C Dillon et al.
ACS chemical biology, 7(7), 1198-1204 (2012-04-18)
Protein arginine methyltransferases (PRMTs) catalyze the posttranslational methylation of arginine using S-adenosylmethionine (SAM) as a methyl-donor. The PRMT family is widely expressed and has been implicated in biological functions such as RNA splicing, transcriptional control, signal transduction, and DNA repair.
Myles B C Dillon et al.
The Journal of biological chemistry, 288(39), 27872-27880 (2013-08-16)
Protein arginine methyltransferase (PRMT) 8 is unique among the PRMTs, as it has a highly restricted tissue expression pattern and an N terminus that contains two automethylation sites and a myristoylation site. PRMTs catalyze the transfer of a methyl group
Neale Harrison et al.
PloS one, 9(11), e113007-e113007 (2014-11-26)
N-acylethanolamines are an important class of lipid signaling molecules found in many species, including the nematode Caenorhabditis elegans (C. elegans) where they are involved in development and adult lifespan. In mammals, the relative activity of the biosynthetic enzyme N-acyl phosphatidylethanolamine-specific

프로토콜

OverExpress™ Chemically Competent Cells

Protocol for OverExpress™ Chemically Competent Cells. Product Numbers: CMC0017, CMC0018, CMC0019, CMC0020, CMC0023, CMC0024

질문

  1. Can C43 competent cells produce periplasmic proteins using pET22b? .

    1 답변

    1. Our lab has not tested pET-22b with CMC0018 C41(DE3) pLysS Chemically Competent Cells. However, according to the following publication, pET-22b seems to work well with C41(DE3) cells: Protein Expr Purif. 2007 Sep;55(1):189-97. doi: 10.1016/j.pep.2007.04.017. Epub 2007 Apr 29. The publication is titled "Cloning and structural analysis of Mycobacterium leprae serine hydroxymethyltransferase" with the PMID: 17540580.

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