CMC0008

STEADY Chemically Competent Cells

Escherichia coli, rod shaped

• UNSPSC 코드: 12352200

속성

• 제품명: STEADY Chemically Competent Cells, for plasmid production using unstable DNA
• 생물학적 소스: Escherichia coli
• Grade: for molecular biology
• 양식: buffered aqueous solution
• 성장 모드: adherent or suspension
• 형태학: rod shaped
• 기술: microbiological culture: suitable
• 세포 형질전환: competent cell type: chemically competent; transformation efficiency: >1 x 10⁸ cfu/μg
• 배송 상태: dry ice
• 저장 온도: −70°C

설명

일반 설명

The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.

Genotype

recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

특징 및 장점

• Clone unstable sequences with reliable results
• Available as chemically competent or electrocompetent cells
• Your choice of two packaging formats
• High efficiency: over 1 × 10⁸ cfu/μg (chemi) or 1 × 10¹⁰ cfu/μg (electro)
• Excellent value

성분

• STEADY chemically competent cells
• pUC 19 transformation control DNA
• recovery medium for cloning

안전성 정보

• Storage Class Code: 10 - Combustible liquids