tsa201 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 96121229, human kidney, Epithelial-like

• 동의어(들): tsA-201 Cells
• UNSPSC 코드: 41106514

속성

• 생물학적 소스: human kidney
• 포장: tube of 5 μg 96121229-DNA-5UG; pkg of vial of cells 96121229-1VL
• 성장 모드: Adherent
• 핵형: Not specified
• 형태학: Epithelial-like
• 제품: Not specified
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human embryonal kidney, SV40 transformed

세포주 설명

tsA201 is a transformed human kidney (293, Sigma catalogue no. 85120602) cell line stably expressing an SV40 temperature-sensitive T antigen. The cell line has been used in a variety of functional expression assays and has been reported to produce high levels of recombinant proteins. Transfection efficiency decreases over a period of several months, therefore it is advisable to lay down a frozen stock as a reserve. The cells have been eradicated from mycoplasma at ECACC. The identity of tsA201 and 293 has been confirmed by STR profiling.

애플리케이션

tsa201 human cell line has been used for protein-binding studies,[1] whole-cell patch clamp recordings[2] and transfection studies.[3]

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 11,12
D13S317: 12
D16S539: 9,13
D5S818: 8,9
D7S820: 11
THO1: 7,9.3
TPOX: 11
vWA: 16,19

배양 배지

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:2 to 1:8; cells are dislodged by mechanical agitation or EDTA solution; 5% CO₂; 37°C. Do not let cells become over confluent as this can affect morphology.

기타 정보

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