Ca Ski

87020501, human cervix (small bowel metastasis), Epithelial

• UNSPSC 코드: 41106514

속성

• product name: Ca Ski, 87020501
• 생물학적 소스: human cervix (small bowel metastasis)
• 성장 모드: Adherent
• 핵형: Modal no. 78
• 형태학: Epithelial
• 제품: Beta Human chorionic gonadotropin (beta-hCG)
• 수용체: Not specified
• 기술: cell culture | mammalian: suitable
• 관련 질환(들): metastasis
• 배송 상태: dry ice
• 저장 온도: −196°C

설명

세포주 기원

Human Caucasian cervical epidermoid carcinoma

세포주 설명

Derived from an epidermoid carcinoma of the cervix metastatic to the small bowel mesentery of a 40 year old Caucasian. Ca Ski cells secrete the β subunit of human chorionic gonadotropin (β-h CG), express tumour-associated antigen and G6PD type B. Ca Ski cells have been reported to contain an integrated human papilloma virus 16 genome (HPV-16 about 600 copies per cell) and HPV-18 related sequences.

애플리케이션

Tumourigenicity studies

DNA 프로파일

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,12
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 7
TPOX: 8
vWA: 17

배양 배지

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

계대배양 정규 작업

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

기타 정보

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