Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
추천 제품
양식
lyophilized powder
기술
affinity chromatography: suitable
Matrix
cross-linked 4% beaded agarose
기질 활성군
cyanate or related structures
기질 스페이서
1 atom (when ligands are coupled via isourea derivative or related linkage)
팽창
1 g swells to 2.5-4.5 mL
용량
≥10 mg/mL binding capacity (BSA)(via amino groups)
적합성
suitable for chromatography
저장 온도
−20°C
유사한 제품을 찾으십니까? 방문 제품 비교 안내
애플리케이션
Cyanogen bromide-activated Agarose is lyophilized powder stabilized with lactose used in affinity chromatography, protein chromatography, protein interactions, antibody labeling, antibody modification and attaching antibodies to agarose beads.
물리적 형태
Lyophilized powder stabilized with lactose
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)
가장 최신 버전 중 하나를 선택하세요:
시험 성적서(COA)
Lot/Batch Number
이미 열람한 고객
Mi Young Cha et al.
Bioconjugate chemistry, 22(1), 88-94 (2010-12-15)
We developed fluorescent biosensor systems that are either general or selective to fluoroquinolone antibiotics by using a single-chain variable-fragment (scFv) as a recognition element. The selectivity of these biosensors to fluoroquinolone antibiotics was rationally tuned through the structural modification on
N A Morjana et al.
Protein expression and purification, 5(2), 144-148 (1994-04-01)
Protein disulfide isomerase (PDI) catalyzes the formation and rearrangement of disulfide bonds during protein folding. PDI coupled to cyanogen bromide-activated agarose retains its catalytic activity, and a column of this material increases both the rate and the yield for folding
Immobilization of solubilized UDP-glucuronosyltransferase from rat liver microsomes to Sepharose 4B.
R A Gilissen et al.
Biochemical pharmacology, 43(12), 2661-2663 (1992-06-23)
A method for the covalent binding of rat liver UDP-glucuronosyltransferase to a cyanogen bromide-activated agarose matrix is described. The rat liver microsomal fraction was solubilized with 8 mM 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate (CHAPS); 90% of the microsomal protein was solubilized. Some 50-60% of
C A Schall et al.
Biotechnology and bioengineering, 53(1), 41-48 (1997-01-05)
The stability of NAD(H) immobilized to a crosslinked agarose support (Sepharose(R)-4B) was examined in buffer solutions at a pH of 7.0 and 8.5. Specifically, this study investigated particle attrition and ligand leakage rates from a cyanogen bromide activated agarose support.
Rodney D Franklin et al.
Obstetrics and gynecology, 101(3), 455-462 (2003-03-15)
To compare the efficacy of unfractionated heparin and low molecular weight heparin in the in vitro binding of antiphospholipid antibodies obtained from the sera of patients with recurrent pregnancy loss. Women with immunoglobulin (Ig) G antibodies to the phospholipids cardiolipin
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What is the Department of Transportation shipping information for this product?
1 답변-
도움이 되었습니까?
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What do you recommend to use as a negative control for Product C9210, Cyanogen bromide?
1 답변-
The control for this product is Product No. CL4B200 (Sepharose CL-4B).
도움이 되었습니까?
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What is the difference between Product C9210, Cyanogen bromide and Product C9142, Cyanogen bromide-activated-Sepharose® 4B?
1 답변-
Sigma-Aldrich produces product C9210, while C9142 is produced by GE Healthcare. In addition, C9210 is a cross-linked resin and C9142 is not.
도움이 되었습니까?
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When using Product C9210, Cyanogen bromide, why must amine containing reagents be avoided during coupling?
1 답변-
The ligand is attached to the resin via an amine group. If an amine-containing buffer is used during coupling, you will greatly reduce the amount of ligand that can attach.
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