추천 제품
생물학적 소스
algae (marine)
Quality Level
형태
suspension
기술
affinity chromatography: suitable
비드(bead) 직경
45-165 μm
공극 크기
30,000-50,000 Da fractionation range (Dextrans)
60,000-20,000,000 Da fractionation range (Globular proteins)
저장 온도
2-8°C
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
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일반 설명
Sepharose™ 4B is an agarose bead and a polymer of Gal β1,4 [3,6]-anhydro-L-galactose. Sepharose aids in fractionating larger molecules and is devoid of charged saccharides. Presence of 3,6-anhydro-L-galactose resists biological degradation. Hydrogen bonds stabilize the sepharose gel structure. Sepharose is widely used in affinity chromatography by cross linking various ligands on the matrix.
애플리케이션
Sepharose™ 4B has been used:
- to conjugate scorpion venom to be used in affinity chromatography
- for clearing of protein extracts prior to immunoprecipitation
- for gel purification and desalting of phospholipid vesicles
- for adsorption of immunoglobulin G (IgG)
법적 정보
Sepharose is a trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point (°F)
100.4 - 109.4 °F - closed cup
Flash Point (°C)
38 - 43 °C - closed cup
Construction of a liposome dialyzer for the preparation of high-value, small-volume liposome formulations
Nature Protocols, 10(6), 927-927 (2015)
Oral Absorption of Mesobuthus eupeus Scorpion Venom in Mice.
Iranian Journal of Toxicology, 11(2), 15-19 (2017)
Principles and Reactions of Protein Extraction, Purification, and Characterization (2004)
PloS one, 14(8), e0220866-e0220866 (2019-08-08)
Dipeptidyl amino-peptidase 3 (DPP3) is an aminopeptidase involved in peptide degradation, including hormone peptides as angiotensin II and enkephalins. DPP3 plasma activity increases in septic patients and correlates with mortality risk. However, the exact physiological role of DPP3 remains unclear
Journal of virology, 81(11), 5579-5593 (2007-03-16)
The human immunodeficiency virus type 1 exterior gp120 envelope glycoprotein is highly flexible, and this flexibility may contribute to the inability of monomeric gp120 immunogens to elicit broadly neutralizing antibodies. We previously showed that an S375W modification of a critical
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