C4879

α-Chymotrypsinogen A from bovine pancreas

essentially salt-free, lyophilized powder

• 동의어(들): chymotrypsin A zymogen
• CAS Number: 9035-75-0
• EC Number: 232-905-3
• MDL number: MFCD00130483
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bovine pancreas
• 유형: Type II
• 양식: essentially salt-free, lyophilized powder
• 특이 활성도: ≥40 units/mg solid
• 분자량: 25,656 Da by calculation
• 정제법: 6× crystallization
• solubility: 1 mM HCl: soluble 10 mg/mL, clear, colorless
• UniProt 수납 번호: P00767
• 외래 활성: α-chymotrypsin ≤1 U/mg (prior to activation by trypsin)
• 저장 온도: −20°C
• 유전자 정보: cow ... CTRB1(618826)

설명

일반 설명

Chymotrypsinogen from bovine pancreas is a zymogen containing 5 disulfide bridges.[1] It has an isoelectric pH of 8.97.

애플리케이션

α-Chymotrypsinogen A from bovine pancreas has been used as model protein crystallization reproducibility studies.[2] It has also been used in the hydrolysis of α-gliadins prior to mass spectroscopy studies.[3]

The enzyme from Sigma has been used in the non-invasive determination of solid-state protein conformation using near infrared (NIR) spectroscopy.[4] It has been used to study the partitioning of protein in polymer/polymer aqueous two-phase systems.[5] The enzyme has also been used for self-interaction chromatography applications, to test the rapid measurement of protein osmotic second virial coefficients. In this technique, the protein is immobilized on chromatographic particles and its retention is measured using isocratic elution.[6]

생화학적/생리학적 작용

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met) on the carboxyl end of the peptide bond.

Chymotrypsinogen A requires limited proteolysis for its activation. Chymotrypsinogen A may be activated by trypsin and chymotrypsin (autolytic activation) to form m α, β, γ, δ and π chymotrypsin (depending upon the conditions of activation).[7] Chymotrypsin is a protease that will preferentially cleave peptides on the carboxyl side of aromatic amino acids including tryptophan, tyrosine, and phenylalanine. It will also hydrolyze peptides on the carboxyl side of leucine, methionine, and alanine.[8]

단위 정의

After activation to Chymotrypsin, one unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

기타 정보

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)